Ultrastructural observations of hippocampal cells in mice infected with Chinese 1 dominant genotype strain of Toxoplasma gondii
10.19405/j.cnki.issn1000-1492.2022.10.010
- Author:
Chong Wang
1
,
2
,
3
;
Yihong Cai
1
,
2
,
3
Author Information
1. Dept of Health Inspection and Quarantine , Anhui Medical University, Hefei 230032
2. Dept of Pathogen Biology and Provincial Laboratories of Pathogen Biology and Zoonoses ,Hefei 230032
3. Anhui Provincial Laboratory of Zoonoses of High Institutions ,Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
hippocampus;
ultrastructure;
mice
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1564-1568
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To observe the effects of TgCtwh3 and TgCtwh6, Toxoplasma gondii of Chinese 1 dominant
genotype, on the ultrastructure of hippocampal of mice.
Methods :Six⁃week⁃old female C57BL/6 mice were randomly divided into three groups: control group, TgCtwh3 infection group and TgCtwh6 infection group, with 6 mice in each group. Control group was intraperitoneally injected with sterile PBS. The TgCtwh3 infected group was intraperitoneally injected with 4 000 TgCtwh3 Toxoplasma gondii tachyzoites and fed for 6 days. The TgCtwh6 infected group was given 20 TgCtwh6 Toxoplasma gondii cysts by gavage and fed for 6 weeks. Then, all the animals were
sacrificed, the brains were obtained for preparing electron microscope specimens and observed.
Results :Compared with the control group, the cells in hippocampal of TgCtwh3 infected group showed nuclear shrinkage and necrosis; cytoplasmic and axon swelling; organelle disappearance; axonotmesis and other pathological changes. Compared with the control group, the cells in hippocampal of TgCtwh6 infected group showed swollen, vacuolated, crista⁃broken or membrane⁃broken mitochondria; lipofuscin deposition; dilation of golgi apparatus and other pathological changes.
Conclusion :Both TgCtwh3 and TgCtwh6 infected mice showed ultrastructural changes in hippocampal compared with normal mice. The TgCtwh3 group showed irreversible cell necrosis, while the TgCtwh6 group showed chronic cell damage mainly caused by mitochondrial structure destruction. These results lay a foundation to explore the pathogenesis of toxoplasmosis.
- Full text:2024073122430358380弓形虫Chinese_1基...鼠海马体细胞超微结构的影响_王崇.pdf
