Ginsenoside RG1 regulates pyruvate kinase M2 on glycolysis and angiogenesis of retinal capillary endothelial cells
10.19405/j.cnki.issn1000-1492.2022.10.009
- Author:
Liping Xue
1
;
Min Hu
1
;
Yadi Li
1
;
Xiaofan Zhang
1
;
Jieying Zhang
1
;
Yuan Zhou
1
;
Jiarui Liang
1
;
Chuanhong Zhang
1
;
Peng Ding
2
Author Information
1. Dept of Pediatric Ophthalmology, Affiliated Hospital ofYunnan University, Kunming 650000
2. Dept of Neurosurgery, The First Affiliated Hospital ofKunming Medical University, Kunming 650000
- Publication Type:Journal Article
- Keywords:
diabetic retinopathy;
GRg1;
HRMECs;
PKM2;
glycolysis;
angiogenesis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1559-1564
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of ginsenoside Rg1 (GRg1) on human retinal microvascular endothelial cells (HRMECs) glycolysis by regulating pyruvate kinase M2 ( PKM2) expression.
Methods :HRMECs were cultured in vitro and divided into normal control (NC) group, high glucose (HG) group, high glucose + ginsenoside Rg1 (HG + GRg1) group, high glucose + ginsenoside Rg1 + low expression PKM2 ( HG + GRg1 + si-PKM2) group, and high glucose + ginsenoside Rg1 + overexpression PKM2 (HG + GRg1 + OE⁃PKM2) group. si-PKM2 and OE⁃PKM2 were transfected into HRMECs cells by cell transfection. The expression of PKM2 mRNA in HRMECs was detected by qRT⁃PCR. The expression levels of related proteins in HRMECs were detected by Western blot. The number of lumen formation in vitro was observed under an inverted microscope to quantify the angiogenesis ability. Cell culture medium of each group was collected, and glucose intake, lactate production and adenosine triphosphate(ATP)content were detected by glucose detection kit, lactate detection kit and ATP detection kit,re spectively.
Results :HG induced HRMECs significantly increased the number of blood vessel formation, glycolysis and PKM2 expression, while GRg1 treatment significantly reduced the number of blood vessel formation, glycolysis and PKM2 expression; transfection of si⁃PKM2 assisted the inhibitory effect of GRg1 on glycolysis and angiogenesis while transfection of OE⁃PKM2 interfered with the function of GRg1 .
Conclusion :GRg1 inhibits angiogenesis by inhibiting PKM2 to reduce glycolysis of HRMECs.
- Full text:202407312239108056人参皂苷RG1调控丙酮酸激...管内皮细胞糖酵解和血管形成_薛黎萍.pdf
