Oligomeric amyloid-β accelerated microglia senescence
- VernacularTitle: 寡聚态β淀粉样蛋白加剧小胶质细胞衰老
- Author:
Zhen WEI
1
;
Xiaoli CUI
1
;
Xiaochun CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: Amyloid-β; Microglia; Cell senescence; Alzheimer’s disease
- From: Journal of Apoplexy and Nervous Diseases 2020;37(8):680-684
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the phenomenon of microglia senescence in vivo and in vitro,and to investigate the effect of amyloid on microglia senescence.Methods Iba-1 immunohistochemical method and senescence-associated β-galactosidase (SA-β-GAL) double staining in the cortex region of brains from young and aged mice was performed for visualizing microglial senescent phenotypes.Purified primary microglia cells were used to observed spontaneous senescence markers at different cultured time point in vitro.Various concentrations of oligomeric Aβ (oAβ) (1-42) were applied to primary microglia cultureto simulate the manifestation.SA-β-GAL staining was used to evaluate the degree of cellular senescence and DNA damage response was explored by γH2AX immunocytochemistry staining.Results Microglia in the aged mouse exhibited aberrant morphological and senescent phenotypes.The increasing rate of SA-β-GAL positive microglia cells was found with the prolongation of culture time in vitro (P<0.001).Compared with the control group,the positive rate of SA-β-GAL staining of microglia cells was increased in the oAβ treatment group (P<0.05).At the same time,the expression of γH2AX was upregulated in the oAβ treated microglial cells (P<0.05).Conclusion These results implied that microglia existed spontaneous senescence phenomenon both in vivo and in vitro,which was contributed to aggravate the pathological aging by oAβ1-42 in primary microglia via the underlying mechanisms of DNA damage response.
- Full text:202407312235005405Oligomeric amyloid-β accelerated microglia senescence.pdf
