Effects of anti⁃NGF and DAPT on nuclear expression of p75NTR and proliferation and invasion ability of esophageal cancer Eca109 cells
10.19405/j.cnki.issn1000-1492.2022.10.005
- Author:
Jianghua Deng
1
;
Qiushi Li
1
;
Bingbing Zhu
1
;
Jingjing Chen
1
;
Xiaoling Mu
1
Author Information
1. School of Medicine , Shihezi University,Key Laboratory of Xinjiang Endemic and Ethnic Diseases ,Shihezi 832000
- Publication Type:Journal Article
- Keywords:
esophageal cancer Eca109 cells;
anti⁃NGF;
γ⁃secretase inhibitor DAPT;
p75NTR
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1536-1541
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effects of the nerve growth factor neutralizing antibody ( anti⁃NGF) and γ⁃secretase inhibitor 3,5⁃difluorophenylacetyl⁃L⁃alanyl⁃S⁃ph ⁃enylglycine⁃t⁃butyl ester (DAPT) on the nuclear expression of p75 neurotrophin receptor (p75NTR ) in esophageal cancer Eca109 cells and on cell proliferation and invasion.
Methods :Immunofluorescence cytochemistry was used to detect the expression changes of p75NTR and Ki6 in Eca109 cells before and after treatment with the anti⁃NGF and γ⁃secretase inhibitor DAPT; CCK⁃8 kit and Transwell cell invasion experiment were used to detect the changes of cell proliferation and invasion ability before and after treatment of Eca109 cells with the anti⁃NGF and γ⁃secretase inhibitor DAPT alone or in combination.
Results:Immunofluorescence cytochemistry showed that after treatment of cells with the anti⁃NGF and γ⁃secretase inhibitor DAPT, the number of cells expressing p75NTR in the nucleus decreased, and after the combined treatment of cells with the anti⁃NGF and γ⁃secretase inhibitor DAPT, the number of cells expressing p75NTR in the nucleus was the least, and the difference was statistically significant (P < 0. 05); CCK⁃8 method and Transwell cell invasion experiment showed that after treatment of cells with the anti⁃NGF and γ⁃secretase inhibitor DAPT, cell proliferation and invasion ability were correspondingly weaker than those before treatment, and the difference was statistically significant (P < 0. 05) .
Conclusion :The anti⁃NGF and γ⁃secretase inhibitor DAPT not only inhibit the nuclear transfer of p75NTR , but also reduce the nuclear expression rate of p75NTR and weaken the cell proliferation and invasion ability accordingly.
- Full text:2024073121580041388NGF中和抗体、DAPT对...及细胞增殖、侵袭能力的影响_邓江华.pdf
