Proteomics analysis of distinct proteins in carotid atherosclerotic plaques
- VernacularTitle:颈动脉粥样硬化斑块的非标记定量蛋白质组学研究
- Author:
Xuan ZOU
1
;
Chen WANG
1
;
Yijing LIU
1
Author Information
- Publication Type:Journal Article
- Keywords: Atherosclerosis ； Plaque ； Proteomics
- From: Journal of Apoplexy and Nervous Diseases 2020;37(9):778-781
- CountryChina
- Language:Chinese
- Abstract: Objective We used proteomics technologies in an attempt to differentiate and identify histological proteins that were associated with carotid atherosclerotic plaques and further understanding of the pathogenesis. Methods Carotid atherosclerotic plaques were obtained from the patients who undergo carotid endarterectomy in the Tianjin Huanhu Hospital. Plaques divided into two groups：stable group and unstable group. We comparatively analyzed the proteome of 10 unstable plaques and 10 stable plaques. The differentially expressed proteins were visualized by two dimensional electrophoresis（2DE）and were analyzed by a coupled label-free and mass spectrometry approach. The protein identification program was used to search the Uniprot. Results A total of 1240 different proteins were identified by label-free and mass spectrometry. A total of 808 proteins were upregulated，and 432 proteins were downregulated in unstable plaques group compared to stable plaques group. They are referring to signal transduction，lipid transport and metabolism，posttranslational modification，cytoskeleton and Proteolysis. Conclusion Proteomics analysis can be used to investigated differentially expressed proteins. However，Additional confirmatory studies are required to elucidated their role in the atherosclerotic process.
- Full text:2024073121473116143Proteomics analysis of distinct proteins in carotid atherosclerotic plaques.pdf
