Preparation and characterization of poly( vinyl alcohol) /bacterial cellulose guided bone regeneration composite film
10.19405/j.cnki.issn1000-1492.2022.10.001
- Author:
Zhengyi Zhao
1
;
Jianhong Xiao
1
;
Chongyuan Liu
1
;
Duohong Zou
1
,
2
,
3
Author Information
1. Stomatological College ofAnhui Medical University, The Affiliated Stomatological Hospital ofAnhui Medical University, Key Lab ofOral Diseases Research ofAnhui Province , Hefei 230032
2. Dept ofOral Surgery, Shanghai Ninth People&prime
3. s Hospital , College ofStomatology, Shanghai Jiao Tong University School ofMedicine , Shanghai Key Laboratory ofStomatology, Shanghai 200001
- Publication Type:Journal Article
- Keywords:
poly (vinyl alcohol);
bacterial cellulose;
solution blending;
biomimetic structural materials
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1513-1517
- CountryChina
- Language:Chinese
-
Abstract:
Objective :By testing the tensile strength of the poly(vinyl alcohol) (PVA)/bacterial cellulose(BC) composite membrane and its effect on the proliferation of mouse embryonic fibroblasts, its potential as new bone tissue engineering membrane were studied.
Methods :PVA⁃BC films of different proportions and pure PVA films were prepared by self⁃evaporation method. The tensile strength of each group was tested. The group with the highest tensile strength was immersed in deionized water for 0. 5 h to measure its wet tensile strength. The microstructure of pure PVA film and the film with the highest tensile strength was observed by scanning electron microscopy (SEM) .X⁃ray diffraction and Fourier transform infrared ( FTIR) spectroscopy were used to analyze pure PVA, pure BC,and the film with the highest tensile strength respectively. Cell counting kit⁃8 (CCK⁃8) was applied to detect the survival rate in the blank control group, the pure PVA film group, and the composite film group with the highest tensile strength.
Results :PVA⁃BC composite films were successfully prepared, X⁃ray diffraction and FTIR analysis revealed the co⁃presence of PVA and bacterial cellulose in the composite film. The initial tensile strength of the composite membrane increased with the BC ratio. When the concentration ratio of PVA to BC was 10 ∶ 7, the tensile strength reached (155. 5 ± 14. 7) MPa, and wet samples reached (13. 8 ± 1. 2) MPa. The CCK⁃8 test of NIH/3T3 showed that there was no significant difference among the PVA⁃BC composite film group, pure PVA group and blank control group after 1,4 and 7 days of cell culture ( P > 0. 05 ) .
Conclusion :PVA⁃BC film fabricated by blending method obtain certain mechanical properties and biocompatibility in both wet and dry states, which may be an appropriate candidate as a GBR membranes for clinical application.
- Full text:2024073121350225289聚乙烯醇-细菌纤维素复合可...屏障膜的制备及理化性能研究_赵正宜.pdf
