Effect of fatty acid binding protein 7 （FABP7） on the permeability of blood-brain barrier in rats with cerebral ischemia-reperfusion
- VernacularTitle:脂肪酸结合蛋白7对脑缺血再灌注大鼠血脑屏障通透性的影响
- Author:
Li CHEN
1
;
Xia ZHE
1
;
Chaochao GU
1
Author Information
- Publication Type:Journal Article
- Keywords: FABP7; Blood-brain barrier; MMP2/9; Cerebral ischemia/reperfusion
- From: Journal of Apoplexy and Nervous Diseases 2020;37(11):1004-1010
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of fatty acid binding protein 7 （FABP7） on blood-brain barrier （BBB） permeability in cerebral ischemia/reperfusion （I/R） rats and the mechanisms involved. Methods BBB cell model was established by co-culture of rat brain microvascular endothelial cells and astrocytes. The BBB model of I/R injury was established by using oxygen-glucose deprivation and reoxygenation （OGD/R） method. This model was treated with the FABP7 recombinant protein （rh-FABP7） alone or together with S7155.ENDOHM instrument，Western blotting and flow cytometry were used to determine the transendothelial electrical resistance （TEER），FABP7，TJ and matrix metalloproteinase （MMP） protein levels，and apoptosis. The rat brain I/R model was established，and rh-FABP7 was injected intraperitoneally. The neurological function score，wet and dry weight method，and Evans blue staining were used to determine the neurological function，brain water content，and BBB permeability of rats. Results FABP7 overexpression reversed the OGD/R-induced down-regulation of TEER，and FABP7 and TJ protein expression，as well as the up-regulation of MMP2/9 expression and apoptosis （P<0.05）. S7155 treatment promoted the effect of FABP7 on MMP2/9 and TJ protein levels，TEER，and apoptosis （P<0.05）. Moreover，FABP7 overexpression reversed the I/R-induced increase of neural function score，brain water content，and BBB permeability and decrease of FABP7 protein level in rats（P<0.05）. Conclusion FABP7 alleviated the damage of brain I/R to BBB integrity by inhibiting MMP2/9.
- Full text:2024073115301322796脂肪酸结合蛋白7对脑缺血再灌注大鼠血脑屏障通透性的影响.pdf
