The time window of osimertinib in the treatment of spinal cord injury in rats
10.19845/j.cnki.zfysjjbzz.2021.0024
- VernacularTitle:奥希替尼治疗大鼠脊髓损伤的时间窗研究
- Author:
Zaiwang LI
1
;
Jingjing WU
1
;
Qian WANG
1
Author Information
1. Department of Neurology,Shenzhen People Hospital (Second Clinical Medical College of Ji nan University First Affiliated Hospital of Southern University of Science and Technology),Shenzhen 518020，Chian
- Publication Type:Journal Article
- Keywords:
Spinal cord injury ； Epidermal growth factor receptor ； Osimertinib ； BBB score ； GAP-43
- From:
Journal of Apoplexy and Nervous Diseases
2021;38(2):100-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the optimal time window to treat spinal cord injury (SCI) by epidermal growth factor receptor (EGFR) inhibitor osimertinib.Methods 60 rats were randomly divided into 6 groups (10 in each group):injury control group and 5 osimertinib-treated groups (0 d,1 d,3 d，5 d and 7 d group).After rats spinal cord injury model was established,the rats in osimertinib-treated group were applied with at different time (0 day,1 day,3 days and 7 days) after injury，while the injury group animals were only given vehicle solution (normal saline) lacking osimertinib.Neurological deficits was evaluated by using the BBB locomotor scale at first day and weekly post-injury.Residual urine volume was recorded everyday in each group.14 days after SCI, 5 rats were randomly selected and sacrificed.Spinal cord tissues were collected,and the expression of GAP-43 was detected by Western blot.Results (1)The BBB score of rats in 0 d group,1 d group,3 d group was significantly higher than 5 d group,7 d group and injury groups(P<0.05),and there was no significant difference among 0 d group,1 d group and 3 d group(P>0.05).(2)The residual urine volume of rats in 0 d group,1 d group,3 d group was significantly less than that other groups (P<0.05),and there was no significant difference among 0 d group,1 d group and 3 d group(P>0.05).(3)The expression of GAP-43 in 0 d group,1 d group and 3 d group was significantly higher than other groups(P<0.05,or P<0.01),and there was no significant difference among 0 d group,1 d group and 3 d group (P>0.05).Conclusion The optimal time of EGFR inhibitor osimertinib to treat spinal cord injury in rats was 0 h to 72 h after spinal cord injury.
- Full text:2024073023040038722The time window of osimertinib in the treatment of spinal cord injury in rats.pdf
