Pathological role of PKC/TRPV1 pathway in trigeminal neuralgia in rats
- VernacularTitle:PKC/TRPV1通路在大鼠三叉神经痛中的病理作用
- Author:
Beibei WU
1
;
Lian SHEN
;
Yuanyin WANG
;
Liecheng WANG
Author Information
- Keywords: PKC; TRPV1; TG; trigeminal neuralgia; mechanical pain threshold
- From: Acta Universitatis Medicinalis Anhui 2024;59(5):846-851
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the pathological role of detect protein kinase C(PKC)/ transient receptor po-tential vanilloid subtype 1(TRPV1) pathway in trigeminal neuralgia (TN) in rats.Methods The infraorbital nerve-chronic constriction injury (ION-CCI) was used to establish a rat model of TN.The rats were randomly di-vided into Sham group,CCI group, CCI+DMSO group and CCI+GF109203X (a PKC inhibitor) group.The me-chanical pain threshold of the rats was measured using a Von Frey brush.qRT-PCR and Western blot were used to detect PKC and TRPV1 in the trigeminal ganglion (TG) .HE staining was used to observe the pathological changes of TG.Results The mechanical pain threshold significantly decreased (P <0.05), and the expression of phos-phorylated PKC(p-PKC) and TRPV1 in TG significantly increased in the CCI group (P<0.05).Histopathological results showed that compared with the Sham group,the CCI group observed significant changes in TG such as in-creased inflammatory cell infiltration and nerve cell swelling.Injection of GF109203X effectively reduced the phos-phorylation of PKC and the expression of TRPV1 in the TG of rats, and the mechanical pain threshold of the rats in-creased (P<0.05) .Under the light microscope, cell swelling and inflammatory cells in the TG were reduced.Conclusion PKC/TRPV1 pathway may be involved in trigeminal neuralgia in rats.
- Full text:202407302203049707PKC_TRPV1通路在大鼠三叉神经痛中的病理作用_吴贝贝.pdf
