Effect of micellar curcumol on polarization of macrophages associated with ovarian cancer
10.19405/j.cnki.issn1000-1492.2024.05.015
- Author:
Qin Tang
1
;
Jing Wang
2
;
Bing Chen
3
;
Sheng Wang
4
;
Minmin Zhang
2
;
Mengyuan Zhang
2
;
Qiang Wu
5
Author Information
1. Dept of Pathology, School of Basic Medical Sciences ,Anhui Medical University, Hefei 230032
2. Dept of Obstetrics and Gynecology,The First Afiliated Hospital of Anhui Medical University,Hefei 230022
3. School of Chemistry and Chemical Engineering , Hefei University of Technology, Hefei 230009
4. Research and Experiment Center, Anhui Medical University, Hefei 230032
5. Dept of Pathology, School of Basic Medical Sciences ,Anhui Medical University, Hefei 230032；Dept of Pathology, The First Afiliated Hospital of Anhui Medical University,Hefei 230022
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
immune microenvironment;
macrophages;
MC;
Akt/mTOR
- From:
Acta Universitatis Medicinalis Anhui
2024;59(5):840-846
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the mechanism of micellar curcumol (MC) regulating the immune microenvironment of ovarian cancer by promoting the polarization of M2⁃type macrophages to M1 ⁃type in ovarian cancer ascites.
Methods :After the mice were divided into groups , a mouse ovarian cancer ascites model was constructed by using the mouse ovarian cancer cell line ID8. Then weight changes were observed , tumor tissue and ascites were collected. The expression of CD86 and CD206 on macrophages of the tumor tissue and ascites was detected by flow cytometry. The expression of protein kinase B (PKB/Akt)/mammalian target of rapamycin ( mTOR) was detected by Western blot. A human monocytic leukemia cell line (THP⁃1) was induced to transform into M2 macrophage ( THP⁃1 M2 macrophage) in vitro , and then treated with 10 μg/ml MC. The apoptosis was detected by flow cytometry. The mRNA levels of macrophage mannose receptor (CD206) , transforming growth factor⁃β (TGF⁃ β) , interleukin (IL) Ⅳ1β and tumor necrosis factor⁃α ( TNF⁃α ) were detected by qRT⁃PCR. The expression of CD86 and CD206 was detected by flow cytometry , and Akt/mTOR expression and phosphorylation was detected by Western blot.
Results :In vitro study showed that the average body weight of the MC group was lower than that of the
control group. Compared with the control group , CD206 expression of macrophages decreased in tumor tissue and ascites in the MC group , while the expression of CD86 increased. The Akt and mTOR phosphorylation level of macrophages in the MC group ′s ascites was lower than that in control group. ② In vivo study showed that there was no difference in apoptosis rate among the groups detected by flow cytometry. The mRNA expression level of CD206 ,TGF⁃ β and the protein expression level of CD206 in MC group were significantly lower than those in the control group , while the mRNA expression of IL⁃1β , TNF⁃α and the protein expression level of CD86 were significantly higher than those in the control group. Compared with the control group , the phosphorylation level of Akt and mTOR in the MC group decreased.
Conclusion :MC promotes M1 polarization of macrophages in ascites to regulate the immune microenvironment of ovarian cancer, which may be related to the Akt/mTOR pathway.
- Full text:2024073021565252074胶束莪术醇对卵巢癌相关巨噬细胞极化的影响_唐勤.pdf
