FTO promotes the development of hepatocellular carcinoma via regulating IFIT2
10.19405/j.cnki.issn1000-1492.2024.05.014
- Author:
Lan Lan
1
;
Zixue Xuan
1
;
Jinying Jiang
1
Author Information
1. Dept of Pharmacy, Center for Clinical Pharmacy , Zhejiang Provincial People s Hospital (Afiliated People s Hospital) , Hangzhou Medical College , Hangzhou 310014
- Publication Type:Journal Article
- Keywords:
FTO;
IFIT2;
hepatocellular carcinoma;
transcriptome sequencing;
HepG2;
m6 A
- From:
Acta Universitatis Medicinalis Anhui
2024;59(5):834-839
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the molecular mechanism of fat mass and obesity⁃associated protein (FTO) regulating hepatocellular carcinoma (HCC) .
Methods:HepG2 cells of knock⁃down FTO were constructed , HepG2 cells of knock⁃down FTO and HepG2 cells were collected , and high⁃throughput sequencing was performed using Illumina Hiseq platform to screen the gene expression differences between the two groups . Through GO and KEGG enrichment analysis of these differential genes , FTO regulatory pathways were studied and downstream target genes of FTO
were screened . The role of FTO downstream target gene in HCC was revealed by bioinformatic analysis and cell experiments .
Results:Transcriptome sequencing showed that 386 genes were differentially expressed between HepG2
cells of knock⁃down FTO and HepG2 cells , and they were involved in biological processes such as response to interferon⁃gamma . The expression of IFIT2 , one of the most responsive interferon⁃stimulating genes , was up⁃regulated after FTO knockdown . Potential m6 A methylation occurred at multiple sites of IFIT2 . The survival of HCC patients with high expression of IFIT2 was significantly prolonged , and knock⁃down of IFIT2 promoted the growth and migration of HepG2 cells .
Conclusion :FTO may regulate IFIT2 by mediating m6 A , and further promote the occurrence and development of HCC .
- Full text:2024073021455588958脂肪量和肥胖相关蛋白FTO...IT2促进肝细胞癌发生发展_兰兰.pdf
