Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1.
10.3347/kjp.2013.51.2.197
- Author:
Wen Hui LI
1
;
Wan Zhong JIA
;
Zi Gang QU
;
Zhi Zhou XIE
;
Jian Xun LUO
;
Hong YIN
;
Xiao Lin SUN
;
Radu BLAGA
;
Bao Quan FU
Author Information
1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of the Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046, Lanzhou, China. fubaoquan@163.com
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Taenia multiceps;
cytochrome c oxidase subunit 1 (cox1);
genotype;
phylogeny;
China
- MeSH:
Animals;
China;
Cluster Analysis;
Cysticercosis/parasitology/veterinary;
DNA, Helminth/chemistry/genetics/isolation & purification;
DNA, Mitochondrial/chemistry/genetics/isolation & purification;
Electron Transport Complex IV/*genetics;
*Genetic Variation;
Goat Diseases/parasitology;
Goats;
Phylogeny;
Polymerase Chain Reaction;
Protein Subunits/genetics;
Sequence Analysis, DNA;
Sheep;
Sheep Diseases/parasitology;
Taenia/*classification/genetics/*isolation & purification
- From:The Korean Journal of Parasitology
2013;51(2):197-201
- CountryRepublic of Korea
- Language:English
-
Abstract:
A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.
