Blood group changes in acute myeloid leukemia and their relationship with therapeutic effect
10.13303/j.cjbt.issn.1004-549x.2024.06.016
- VernacularTitle:急性髓系白血病血型变化及其与治疗效果的关系
- Author:
Qianqian SONG
1
;
Shuang YANG
;
Qianmeng HAO
;
Yulin ZHANG
Author Information
1. 郑州大学第二附属医院 输血科,河南 郑州 450000
- Keywords:
forward and reverse typing discrepancy;
acute myeloid leukemia;
therapeutic effect;
weakened antigenicity of the ABO blood group;
gene sequencing
- From:
Chinese Journal of Blood Transfusion
2024;37(6):700-704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the blood group changes of two acute myeloid leukemia patients with suspected O type,and their relationship with the therapeutic effect.Methods Serological analysis of ABO blood group of patients was carried out by microcolumn gel method,tube method and absorption-elution test,ABO blood group genotyping was per-formed by microfluidic chip method.Exons E2 to E7 of ABO gene were amplified by PCR and sequenced by Sanger method.Results The forward typing of two cases were both O type,but the reverse typing were both A type.The absorption-elution test results all showed detection of antigen A.ABO gene phenotype of the two cases were both A,with genotyping results as A102/A102 and A102/O01,respectively.Sequencing results showed that SNP sites of ABO blood group were 467T/T,261G/delG and 467C/T,respectively.In one case,the intensity of anti-A agglutination reaction changed significantly from weak to strong with the progress of treatment.Conclusion For clinical samples of acute myeloid leukemia patients with ABO for-ward and reverse typing discrepancy and suspected O type,the result of reverse typing should be valued,and absorption-e-lution test should be performed to further confirm the ABO blood type combining the genetic test results,so as to develop ap-propriate blood transfusion strategies for patients.
