Occult HBV among anti-HBc+alone and not alone samples:mutation analysis of S Gene
10.13303/j.cjbt.issn.1004-549x.2024.07.013
- VernacularTitle:OBI感染中单独抗-HBc+和非单独抗-HBc+献血者S区变异情况分析
- Author:
Jianxun ZHENG
1
;
Shiquan WU
;
Zhenzhen LIU
;
Sujun JIANG
Author Information
1. 衢州市中心血站,浙江 衢州 324000
- Keywords:
occult hepatitis B virus;
anti-HBc+;
mutation site
- From:
Chinese Journal of Blood Transfusion
2024;37(7):797-801
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the status of occult hepatitis B virus infection(OBI)among blood donors in Quzhou,Zhejiang,and to analyze the mutation of S region in blood donors with anti-HBc+alone and non anti-HBc+alone.Methods The OBI samples were screened by ELISA and NAT;the HBV DNA was amplified and sequenced;20 anti-HBc+alone and 25 not anti-HBc+alone samples were obtained.Results The detection rate of OBI in Quzhou was 0.10%(155/161 045),and the positive rate of anti-HBc was 74.19%(115/155).The detection rate of OBI increased with the age of blood donors(P＜0.05),but was not related to gender.The positive rate of anti-HBc+alone was 22.58%(35/155),and that of not anti-HBc alone was 51.61%(80/155).Among the 45 OBI sequencing samples,the proportion of B and C geno-type was73.33%(33/45)and 20.00%(9/45),respectively.The mutation sites of blood donors in the anti-HBc+alone group were more than those in the not anti-HBc+alone group,and the mutation rates of S114T and V168A on MHR were significantly different(P＜0.05).Conclusion The genotype of OBI infection in Quzhou is mainly type B.The mutation sites of blood donors with anti-HBc+alone are higher than those with not anti-HBc+alone,which may be more suitable as one of the OBI screening indicators.
