Occult HBV among anti-HBc + alone and not alone samples: mutation analysis of S Gene
10.13303/j.cjbt.issn.1004-549x.2024.07.013
- VernacularTitle:OBI感染中单独抗-HBc＋和非单独抗-HBc＋献血者S区变异情况分析
- Author:
Jianxun ZHENG
1
;
Shiquan WU
1
;
Zhenzhen LIU
1
;
Sujun JIANG
1
Author Information
1. Quzhou Central Blood Station, Quzhou 324000, China
- Publication Type:Journal Article
- Keywords:
occult hepatitis B virus;
anti-HBc+;
mutation site
- From:
Chinese Journal of Blood Transfusion
2024;37(7):797-801
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the status of occult hepatitis B virus infection(OBI) among blood donors in Quzhou, Zhejiang, and to analyze the mutation of S region in blood donors with anti-HBc+ alone and non anti-HBc+ alone. 【Methods】 The OBI samples were screened by ELISA and NAT; the HBV DNA was amplified and sequenced; 20 anti-HBc+ alone and 25 not anti-HBc+ alone samples were obtained. 【Results】 The detection rate of OBI in Quzhou was 0.10%(155/161 045), and the positive rate of anti-HBc was 74.19%(115/155). The detection rate of OBI increased with the age of blood donors(P<0.05), but was not related to gender. The positive rate of anti-HBc+ alone was 22.58%(35/155), and that of not anti-HBc alone was 51.61%(80/155). Among the 45 OBI sequencing samples, the proportion of B and C genotype was 73.33%(33/45) and 20.00%(9/45), respectively. The mutation sites of blood donors in the anti-HBc+ alone group were more than those in the not anti-HBc+ alone group, and the mutation rates of S114T and V168A on MHR were significantly different(P<0.05). 【Conclusion】 The genotype of OBI infection in Quzhou is mainly type B. The mutation sites of blood donors with anti-HBc+ alone are higher than those with not anti-HBc+ alone, which may be more suitable as one of the OBI screening indicators.
