The Dimension of Trichomonas vaginalis as Measured by Scanning Electron Microscopy.
10.3347/kjp.2013.51.2.243
- Author:
Sang Hoon CHEON
1
;
Seung Ryong KIM
;
Hyun Ouk SONG
;
Myoung Hee AHN
;
Jae Sook RYU
Author Information
1. Department of Obstetrics and Gynecology, Hanyang University Guri Hospital, College of Medicine, Guri 471-701, Korea.
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Trichomonas vaginalis;
size;
axostyle;
scanning electron microscopy
- MeSH:
*Biometry;
Female;
Humans;
*Microscopy, Electron, Scanning;
Organelles/ultrastructure;
Trichomonas Infections/parasitology;
Trichomonas vaginalis/*cytology/isolation & purification/*ultrastructure
- From:The Korean Journal of Parasitology
2013;51(2):243-246
- CountryRepublic of Korea
- Language:English
-
Abstract:
It is known that physicochemical conditions (e.g., pH, temperature, and ionic strength) affect the size of trichomonads. In this study, the sizes of 4 isolates of Trichomonas vaginalis cultured for more than a year (called "old T") and 3 isolates freshly isolated from vaginitis cases (called "fresh T") were compared by scanning electron microscopy. Although the fresh T had shorter body length, body width, and flagellar length than old T, total length (about 26 microm), including body length, flagella length, and axostyle length was almost the same in the 2 groups. A striking difference was observed between the axostyles of the 2 groups; the axostyle length of the fresh T (8.2 microm) was more than twice as long as that of the old T (4.0 microm). However, in several parasitology textbooks, the length of T. vaginalis is said to vary widely from 7 to 32 microm, and its undulating membrane is said to extend about half way (53.5%) to the posterior end of the body. On the other hand, in our study, the undulating membrane was observed to extend more than 3/4 of the body length (72.1%) in old T, whereas in fresh T it could not be measured. Taken together, we suggest that T. vaginalis averages 26 (21-32) microm in total length, with 9.5 (7.4-11.4) microm of body length and 6.8 (5.3-7.7) microm of width, and its undulating membrane extending 3/4 of its body length. Therefore, these findings may provide useful information for morphological characteristics of T. vaginalis.
