Ox-LDL simulates biosynthesis of resolvin D2 by human periphery blood mononuclear cells-derived macrophages
10.19845/j.cnki.zfysjjbzz.2021.0130
- VernacularTitle: Ox-LDL刺激人外周血单个核细胞来源巨噬细胞RvD2的合成
- Author:
Xin TANG
1
;
Xiuzhe WANG
1
;
Yuwu ZHAO
1
Author Information
1. Department of Neurology,Shanghai Jiao Tong University Affiliated Sixth People Hospital,Shanghai 200233,China
- Publication Type:Journal Article
- Keywords:
RvD2;
Macrophage;
ox-LDL;
Ischemic stroke;
Inflammation
- From:
Journal of Apoplexy and Nervous Diseases
2021;38(6):484-487
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of ox-LDL on the biosynthesis of resolving D2 (RvD2) by human periphery blood mononuclear cells (PBMCs)-derived macrophages in ischemic stroke (IS).Methods Matured macrophages were derived from PBMCs of ischemic stroke patients and healthy controls,and were grouped to vehicle group and oxidized low-density lipoprotein (ox-LDL) group.We compared the level of RvD2,15-lipoxygenase (15-LOX) and 5-lipoxygenase (5-LOX),as well as G-protein coupled receptor 18 (GPR18).between groups,and determined the formation of foam cells.Results Compared with vehicle group,the level of RvD2(P<0.001),15-LOX and GPR18(P<0.05) in ox-LDL stimulated group was significantly increased,however,no significant difference in the expression of 5-LOX between ox-LDL and vehicle group was observed.After ox-LDL stimulation,compared with healthy control,elevation of RvD2 synthesis in ischemic stroke group was significantly lower（P<0.05）,and foam cell formation was also more severe in macrophages of stroke patients.Conclusion Ox-LDL stimulates biosynthesis of RvD2 by human macrophage,perhaps through enhancing 15-LOX expression.Moreover,function of increasing RvD2 synthesis upon ox-LDL stimulation is impaired in stroke patients.
- Full text:2024072921480467419Ox-LDL simulates biosynthesis of resolvin D2 by human periphery blood mononuclear cells-derived macrophages.pdf
