Expression characteristics of CB1R in hippocampal neurotransmission loop after status epilepticus in rats
- VernacularTitle:大鼠癫痫持续状态后海马神经传递环路CB1R表达特征
- Author:
Xiandong MENG
1
;
Haifeng ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: Epilepsy ； Cannabinoid type I receptor ； Hippocampus ； Sclerosis
- From: Journal of Apoplexy and Nervous Diseases 2021;38(7):591-595
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the changes in CB1R expression in various regions of the hippocampus of rats at different time points after status epilepticus.Methods Use lithium chloride-pilocarpine to induce status epilepticus（SE）in rats.At 1 w，2 w，3 w，and 4 w after SE，rat hippocampal slices were taken，and the cannabinoid type 1 receptor （CB1R） immunohistochemical staining was performed to measure the CA1 stratum radiatum，CA-3 stratum lucidum，and stratum moleculare CB1R mean integrated optical density value（MIOD），all values are statistically analyzed. At the same time，hippocampal slices were taken 4 w after SE and stained with NeuN and GFAP to observe the changes of hippocampal neurons and astrocytes.Results In the stratum radiatum of hippocampal CA-1 area，the expression of CB1R at each time point after SE was higher than that of the control（P<0.05），and the expression of CB1R 4 w after SE was higher than 1 w，2 w，3 w after SE （P<0.05）；In the stratum lucidum of hippocampal CA-3 area，the expression of CB1R at each time point after SE was higher than that of the control （P<0.05），and the expression of CB1R 4W after SE was higher than 1 w after SE （P<0.05）；In the hippocampus stratum moleculare，the expression of CB1R at each time point after SE was higher than that of the control（P<0.05），but the expression of CB1R was not statistically different between 1 w，2 w，3 w，and 4 w after SE （P>0.05）. Neurons in the stratum pyramidale of the hippocampal CA-1 area were significantly lost，and astrocytes proliferated and enlarged 4 w after SE.Conclusion Rats had the characteristics of sclerotic hippocampus at 4 w after SE. After SE，the expression of CB1R in stratum radiatum of CA1 area，stratum lucidum of CA3 area，and stratum moleculare of the rat hippocampus increased. In the CA1 area’s stratum radiatum and CA3 area’s stratum lucidum CB1R increased most significantly at 4 w after SE.The stratum moleculare CB1R increased at 1 w after SE and the increase was not obvious later.
- Full text:20240729204506261772j.cnki.zfysjjbzz.2021.pdf
