Establishing and identification of neurovascular unit in a tri-culture model using microfluidic chip
10.19845/j.cnki.zfysjjbzz.2021.0234
- VernacularTitle: 以微流控芯片为载体的神经血管单元三细胞共培养模型的构建与鉴定
- Author:
Han LUO
1
;
Weiliang SHU
1
;
Chuan CAI
1
Author Information
1. Shenzhen Longhua District Central Hospital,Shenzhen 518110,China
- Publication Type:Journal Article
- Keywords:
Neurovascular unit;
Tri-culture;
Cell growth curves;
Cell viability;
Microfluidic chip
- From:
Journal of Apoplexy and Nervous Diseases
2021;38(10):1070-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective We established a model by using microfluidic chip that is a horizontal side by side diffusion system through microporous separating three adjacent channels in a triple-culture using neurons,astrocytes,and bEnd.3.Methods (1)In the in vitro microphysiological system of neurovascular unit,we identified the seed density,seed time,seed sequence and seed method.(2)We adopt the repeated measures data of ANOVA to see whether there is significant difference in the viability when the three cell types are tri-cultured and separately mono-cultured.Results (1) In the novel in vitro NVU model,the seed density of the neurons,astrocytes and bEnd.3 is 5×10^6~10×10^6 /ml,3×10^5~8×10^5个/ml and 1×10^5~5×10^5/ml.The seed sequence is from neurons to astrocytes to bEnd.3.The seed time is that after the previous seeded cells enter the exponential phase,the next cells will be seeded.(2)We adopt the repeated measures data of ANOVA to see whether there is significant difference in the viability when the three cell types are tri-cultured and separately mono-cultured.Conclusion In this study,neurons,astrocytes and bEnd.3 were tri-cultured by using microfluidic chip.In this tri-culture model,these three kinds of cells have high cell viability.
- Full text:20240729090652129381Establishing and identification of neurovascular unit in a tri-culture model using microfluidic chip.pdf
