Study on characteristic chromatogram of Yao medicine Kadsura longipedunculata and its anwulignan content and anti-inflammatory activity
- VernacularTitle:瑶药小钻的特征图谱及其安五脂素含量和抗炎活性研究
- Author:
Binglan TANG
1
;
Wei GAO
2
;
Chengjian ZHAO
1
;
Chunli OU
1
;
Xiaoli HOU
1
;
Lu CHEN
1
;
Dandan MO
1
Author Information
1. National Traditional Chinese Medicine Inheritance and Innovation Center，Guangxi Botanical Garden of Medicinal Plant/ Guangxi Key Laboratory of Medicinal Resource Protection and Genetic Improvement/Guangxi Zhuang Autonomous Region Traditional Chinese Medicine Resources Intelligent Creation Engineering Research Center，Nanning 530023，China
2. Guangxi Zhuang Autonomous Region Drug Administration，Nanning 530029，China
- Publication Type:Journal Article
- Keywords:
Yao medicine Kadsura longipedunculata;
HPLC;
characteristic chromatogram;
quality control;
anwulignan;
anti-
- From:
China Pharmacy
2024;35(14):1727-1731
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish characteristic chromatogram of Yao medicine Kadsura longipedunculata and the method for the content determination of its main component anwulignan， and evaluate the anti-inflammatory activity of anwulignan. METHODS HPLC method was performed with acetonitrile-0.5% phosphoric acid solution as the mobile phase for gradient elution. The characteristic chromatogram of K. longipedunculata was established and similarity was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM （2012 edition）. The content of anwulignan in K. longipedunculata was determined. Lipopolysaccharide induced RAW264.7 macrophages were selected as inflammatory cell model to investigate the effects of anwulignan on the levels of tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β） and IL-6. RESULTS The similarities of characteristic chromatogram for 10 batches of K. longipedunculata ranged 0.901-0.994， and 9 common peaks were determined； 3 components were identified， such as changnan schisantherin E， kadsulactone A， anwulignan. The contents of anwulignan were （0.72±0.05）-（1.21±0.03） mg/g（n＝3）. Anwulignan of 0.125-0.5 μg/mL greatly decreased the levels of TNF-α， IL-1β and IL-6 in the supernatant of inflammatory model cells （P＜0.05 or P＜0.01）. CONCLUSIONS HPLC characteristic chromatogram of K. longipedunculata and the method for the content determination of anwulignan are all established， and anwulignan may be the active ingredient of anti-inflammatory effect in K. longipedunculata.
