Expert consensuses on the application of nanopore sequencing technology in the detection of pathogenic micro-organisms

Shuyao Zhang; Tieying Hou; Xiaoyan LI; Shilong Zhong; Junyan WU; Bin Huang; Society Division; Association Expert; Microorganisms The

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Expert consensuses on the application of nanopore sequencing technology in the detection of pathogenic micro-organisms

VernacularTitle:纳米孔测序在病原微生物检测中的应用专家共识
Author: Shuyao ZHANG ¹ ; Tieying HOU ² ; Xiaoyan LI ³ ; Shilong ZHONG ⁴ ; Junyan WU ⁵ ; Bin HUANG ⁶ ; Society DIVISION ⁴ ; Association EXPERT ⁵ ; Microorganisms THE ⁶
Author Information

1. Clinical Precision Treatment Center， the Affiliated Guangzhou Red Cross Hospital， Jinan University， Guangzhou 510240， China
2. Huazhong University of Science and Technology Union Hospital （Nanshan Hospital），Guangdong Shenzhen 518101， China
3. Dept. of Pharmacy， the Sixth Affiliated Hospital of Sun Yat-sen University， Guangzhou 510510， China
4. Dept. of Pharmacy， Guangdong Provincial People’s Hospital，Guangzhou 519041， China
5. 5. Dept. of Pharmacy， Sun Yat-sen Memorial Hospital， Sun Yat-sen University，Guangzhou 510120， China
6. Dept. of Clinical Laboratory， the First Affiliated Hospital of Sun Yat-sen University， Guangzhou 510080， China
Publication Type:Journal Article
Keywords: third-generation sequencing; nanopore sequencing; pathogenic microorganisms; critical infection; clinical laboratory tests; microbiological diagnosis
From: China Pharmacy 2024;35(14):1673-1731
CountryChina
Language:Chinese
Abstract: OBJECTIVE To improve the diagnosis and treatment level of critically ill infectious diseases， standardize the clinical application of nanopore sequencing and promote the sound development of the technology. METHODS Division of Therapeutic Drug Monitoring of Chinese Pharmacological Society and Expert Committee of Precision Medicine for Clinical Treatment of Guangdong Pharmaceutical Association initiated and organized multidisciplinary experts to discuss and determine the consensus writing outline by using the nominal group method， forming a preliminary consensus draft； expert consultation was performed by using Delphi method， and then experts’ opinions were analyzed and revised to form consensus. RESULTS & CONCLUSIONS Consensuses of Experts on the Application of Nanopore Sequencing Technology in the Detection of Pathogenic Microorganisms covers targeted sequencing， metagenomic sequencing and whole genome sequencing， and is standardized in terms of sample collection and storage， detection process， bioinformatics analysis and report interpretation； the recommendations are provided for the key issues.