Role of hippocampal p75 neurotrophin receptor in postoperative cognitive dysfunction of aged mice
10.19845/j.cnki.zfysjjbzz.2021.0286
- VernacularTitle:海马p75神经营养因子受体在老年小鼠术后认知障碍中的作用
- Author:
Yuzhu GAO
1
;
Muhuo JI
1
;
Zhiqiang ZHOU
1
Author Information
1. Department of Anesthesiology,Jinling Hospital,School of Medicine,Nanjing University,Nanjing 210002,China
- Publication Type:Journal Article
- Keywords:
Aging;
POCD;
Hippocampus;
p75NTR
- From:
Journal of Apoplexy and Nervous Diseases
2021;38(12):1072-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of the p75 neurotrophic receptor (p75 NTR) in the cognitive impairment induced by laparotomy surgery of aged mice,and to explore the possible mechanism.Methods Thirty eighteen-month-old male mice were equally randomized into three groups (n=10):control group,surgery group,surgery+TAT group.Open field test was performed 5 days after the surgery to record the exploration distance and the time in the center.Fear-conditioned training was performed 6 days after the surgery,and fear-conditioned test was performed 7 days after the surgery to record the freezing time.The hippocampus was harvested immediately after the behavioral test.Western blot was applied to detect the levels of p75NTR,BDNF,PSD95,TrkB and pCREB in the hippocampus,immunofluorescence was applied to detect the number of caspase-3 positive cells in the hippocampus,and Golgi staining was applied to observe the changes of dendritic spines in hippocampal CA-1 region.Results In the open field test,the total distance and the time in the center had no significant difference among the three groups (P>0.05).In the fear conditioning test,compared with control group,the percentage of the contextual conditional freezing time decreased significantly in surgery group (P<0.05),and the expressions of BDNF，PSD95，TrkB and pCREB and the number of dendritic spines in CA-1 were significantly decreased (P<0.05),the expression of p75NTR and the number of caspase-3 positive cells were increased significantly in the surgery group (P<0.05).Compared with the surgery group,the percentage of the contextual conditional freezing time increased significantly in surgery+TAT group,and the expressions of BDNF,PSD95,TrkB,and pCREB and the number of dendritic spines in CA-1 were significantly increased,the number of caspase-3 positive cells were decreased significantly in the surgery+TAT group (P<0.05).Conclusion Hippocampal p75 NTR plays a key role in the pathophysiology of POCD.
- Full text:20240728215622757121Role of hippocampal p75 neurotrophin receptor in postoperative cognitive dysfunction of aged mice.pdf
