The protective mechanisms of hippocampal neurons on RhoA/ROCK2 pathway mediated by Rho kinase inhibitors in vascular dementia rats
10.19845/j.cnki.zfysjjbzz.2021.0283
- VernacularTitle:Rho激酶抑制剂介导RhoA/ROCK2通路对VaD大鼠海马神经元的保护机制
- Author:
Yueqiang HU
1
;
Junjun XIANG
1
;
Qiumei DENG
1
Author Information
1. Department of Neurology,The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China
- Publication Type:Journal Article
- Keywords:
Vascular dementia； Synaptic regeneration； Adeno-associated virus ； Rho kinase inhibitors ； RhoA/ROCK2 pathways
- From:
Journal of Apoplexy and Nervous Diseases
2021;38(12):1060-1063
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective mechanism of Rho protein kinase 2 (ROCK2) inhibitor constructed based on adeno-associated virus vector on vascular dementia (VaD) rat hippocampal neurons.Methods Forty SD rats were randomly divided into sham operation group,model group,ROCK2 interference group (shROCK2),negative control group.The VaD model was prepared by permanent ligation of bilateral common carotid arteries.AAV9-ROCK2-shRNA was injected into the hippocampus of the rat by stereotaxic technique,the negative control group was injected with the same amount of empty vector adeno-associated virus.After 4 weeks,morris water maze was used to test the learning and memory abilities of rats.RT-PCR and Western blot were used to detect the mRNA and protein expression levels of RhoA,ROCK2,MLC,MLCP.Results Four weeks after operation.The mRNA and protein expressions of the above 4 indicators in hippocampus increased (P<0.01),the synaptic vesicles disappeared,and the structure of hippocampus was chaotic in the model group.Compared with the model and control group,the learning and memory ability of rats in ROCK2 interference group was significantly improved,the mRNA and protein of the above 4 indicators were significantly decreased (P<0.05),and the pre-synaptic vesicles were normal and the organelles were more complete.Conclusion Rho protein kinase 2 inhibitors can reduce the expression of downstream related proteins by inhibiting RhoA/ROCK2 signaling pathway,then promote neuronal axon regeneration and alleviate cognitive dysfunction of VaD rats.
- Full text:2024072821464777988The protective mechanisms of hippocampal neurons on RhoA_ROCK2 pathway mediated by Rho kinase inhibitors in vascular dementia rats.pdf
