Erastin induces ferroptosis in lung fibroblasts through MAPK mediated oxidative stress signaling pathway
10.19405/j.cnki.issn1000-1492.2024.05.012
- Author:
Yiran Wang
1
;
Shijie Zhang
1
;
Yubo Guan
2
;
Miaomiao Li
2
;
Ruyi Cai
3
;
Qi Wu
1
Author Information
1. Dept of Physiology, Xuzhou Medical University, Xuzhou 221004
2. Dept of Pathophysiology, Xuzhou Medical University, Xuzhou 221004
3. The First School of Clinical Medicine , Xuzhou Medical University, Xuzhou 221004
- Publication Type:Journal Article
- Keywords:
pulmonary fibrosis;
lung fibroblasts;
ferroptosis;
reactive oxygen species;
MAPK signaling pathway
- From:
Acta Universitatis Medicinalis Anhui
2024;59(5):820-825
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the mechanism by which Erastin affects ferroptosis in lung fibroblasts.
Methods:Mouse lung fibroblasts (C57/B6⁃L) were treated with varying concentrations of the iron death inducer Erastin, Cell viability was assessed using the cell counting Kit⁃8 (CCK⁃8) assay. Oxidative stress levels were visualize using a fluorescence microscope , and the expression of proteins related to the mitogen⁃activated protein kinase (MAPK) signaling pathway was analyzed using Western blot. Additionally , the p38 and extracellular regulated protein kinase (ERK) inhibitors SB203580 and U0126 were employed to further elucidate the mechanism by which Erastin induces iron death in lung fibroblasts.
At a concentration of 100 μmol/L , Erastin effectively in⁃duced ferroptosis in lung fibroblasts , leading to an upregulation of oxidative stress. Furthermore , the phosphoryla⁃tion levels of p38 and ERK proteins in the MAPK pathway were elevated (P < 0. 05) . The addition of SB203580 and U0126 inhibitors resulted in a significant reduction in oxidative stress levels and a notable increased in cell actiivity in lung fibroblasts (P < 0. 05) .
It can be concluded that Erastin induces ferroptosis in lung fibroblasts , potentially through the mediation of oxidative stress via the MAPK signaling pathway.
- Full text:2024072815272956550Erastin通过MAPK...通路诱导肺成纤维细胞铁死亡_王沂然.pdf
