Molecular characterization and tissue expression of g ⁃type lysozyme in glytothorax zainaensis
10.19405/j.cnki.issn1000-1492.2023.05.002
- Author:
Yunjiao Wang
1
;
Meng Lv
1
;
Shasha Cai
1
Author Information
1. Graduate School ,Shandong First Medical University(Shandong Academy of Medical Sciences) ,Jinan 250000
- Publication Type:Journal Article
- Keywords:
G. zainaensis;
g ⁃type lysozyme;
bioinformatics analysis;
tissue expression profile
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):711-717
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify and characterize the g ⁃type lysozyme in G. zanaensis and analyze its role in host immune system.
Methods:GzLysG cDNA sequence was cloned by Nest PCR technology. Bioinformatics analysis of the GzLysG protein was carried out by ExPASy , SignalP 5. 0 , CDD , Cluster Omega and other online software. The GzLysG gene expression pattern in G. zanaensis tissues was detected by RT⁃qPCR.
Results:The results showed that open reading frame of GzLysG cDNA was 558 bp in length , encoding 185 amino acids. The molecular weight and theoretical isoelectric point of the reduced protein was 20 478. 20 and 9. 16 , respectively. GzLysG was predicted to be a basic and hydrophilic protein , had no signal peptide and contained the typical catalytic active site , GEWL domain and SLT domain of g ⁃type lysozyme. Advanced structural analysis revealed that GzLysG protein which was closely related to lysozyme activity. Lysozyme was highly conserved in evolution , with GzLysG showing a close topologic relationship with lysozyme from Danio rerio. Quantitative real⁃time polymerase chain reaction analysis indicated that GzLysG ubiquitously existed in all examined tissues , with higher mRNA expression levels observed in skin , muscle and gill.
Conclusion:All those results suggest that GzLysG plays a key role in G. zanaensis immune defensive system.
- Full text:2024072722100928096扎那纹胸鮡g型溶菌酶的cDNA克隆及组织表达分析_王运娇.pdf
