The value of combined SDC2 and TFPI2 gene methylation testing in the early screening of colorectal cancer
10.19405/j.cnki.issn1000-1492.2023.04.027
- Author:
Mengzhen Chu
1
;
Yigui Tang
1
;
Min Zhang
1
;
Yuanyuan Hu
1
;
Jiadan Xu
1
;
Yang Zhang
1
;
Yuping Yang
1
;
Anyong Wang
1
;
Zhongxin Wang
1
Author Information
1. Dept of Clinical Laboratory，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
methylation;
SDC2 gene;
TFPI2 gene
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):682-686
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the value of combined stool syndecan-2 (SDC2) and tissue factor pathway inhibitor 2 ( TFPI2) gene methylation testing in the early screening of colorectal cancer．
Methods :106 patients with colorectal cancer (colorectal cancer group) ，75 patients with advanced adenoma ( advanced adenoma group) and 35 patients with non-advanced adenoma (non-advanced adenoma group) were selected as study subjects，and 153 patients with other gastrointestinal disorders and 182 patients with negative colonoscopy results during the same period were selected as the control group．The quantitative methylation-specific PCR(qMSP) method was used to detect SDC2 and TFPI2 gene methylation in the stool specimens of all subjects．The sensitivity and specificity of the combined SDC2 and TFPI2 gene methylation assay for the detection of colorectal cancer and adenoma were evaluated using colonoscopy and pathology results as the gold standard.
Results :Among 106 patients with colorectal cancer， the sensitivity of combined methylation test was 93. 4% ; among 75 patients with advanced adenoma，the sensitivity of combined methylation test was 62. 7% ; among 35 patients with non-advanced adenoma，the sensitivity of combined methylation test was 34. 3% ; the specificity of the combined SDC2 and TFPI2 gene methylation test for colorectal cancer and adenoma screening was 94. 6%．
Conclusion:The combined SDC2 and TFPI2 gene methylation test has high sensitivity for colorectal cancer and its early lesions，and it also maintains high specificity.
- Full text:2024072720114668289SDC2和TFPI2基因甲...直肠癌早期筛查中的应用价值_储梦真.pdf
