Effects of estrogen deficiency on depression-like behavior and hippocampal mitochondrial function in mice
10.19405/j.cnki.issn1000-1492.2023.04.025
- Author:
Mengruo Jiang
1
;
Yongjun Xu
2
;
Liyuan Wang
3
;
Hui Sheng
3
Author Information
1. 1th Battalion Student Brigade，College of Basic Medicine Naval Medical University，Shanghai 200433
2. Basic Medical Laboratory，The 900th Hospital of Joint Logistics Support Force，PLA，Fuzhou 350025
3. Dept of Physiology，College of Basic Medicine Naval Medical University，Shanghai 200433
- Publication Type:Journal Article
- Keywords:
mitochondria;
depression;
estrogens
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):672-675
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of estrogen deficiency on depression-like behavior and mitochondrial function in hippocampus，and to study the underlying mechanisms．
Methods:The female adult C57BL /6 mice were randomly assigned to Sham group，Ovx group and Ovx + E2 group．On the 7th day after operation，Ovx + E2 group was subcutaneously injected with 17 β-estradiol ( E2 ) for 4 weeks．Then another 48 female adult C57BL /6 mice were randomly divided into Sham group，Sham + MnTBAP group，Ovx group and Ovx + MnTBAP group．After operation for one week，MnTBAP was injected into the hippocampus of mice in Sham + MnTBAP group and Ovx + MnTBAP group．The depression-like behaviors of the mice were monitored by sucrose preference test and forced swimming test．Then the mice were decapitated，and the hippocampus samples were collected．Mitochondria of hippocampus were isolated using mitochondria fractionation kit．The concentrations of adenosine triphosphate (ATP) ， reactive oxygen species ( ROS) and mitochondrial membrane potential were determined，respectively.
Results:Compared with Sham mice，Ovx showed a decrease in the percentage of sucrose consumption，an increase in immobility time and the alterations of hippocampal mitochondrial function，which were reversed by estrogen treatment. Intrahippocampal injection of MnTBAP significantly increased the percentage of sucrose consumption and decreased immobility time in Ovx mice．
Conclusion:Hippocampal mitochondrial dysfunction contributes to estrogen deficiency-induced depression-like behaviors.
- Full text:2024072720000112869雌激素缺乏对小鼠抑郁样行为和海马线粒体功能的影响_蒋梦若.pdf
