Involvement of NLRP3 inflammasome in hyperalgesia induced by temporomandibular joint osteoarthritis
10.19405/j.cnki.issn1000-1492.2023.04.022
- Author:
Miaomiao Bai
1
;
Zhenguo Shen
1
;
Chunmeng Ding
1
;
Tian Xing
1
;
Aibing Hou
1
Author Information
1. Stomatological College of Anhui Medical University，The Affiliated Stomatological Hospital of Anhui Medical University，Key Lab of Oral Diseases Research of Anhui Province，Hefei 230032
- Publication Type:Journal Article
- Keywords:
NLRP3 inflammasome;
temporomandibular joint osteoarthritis;
hyperalgesia;
trigeminal ganglion
- From:
Acta Universitatis Medicinalis Anhui
2023;58(4):655-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of nucleotidebinding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome in hyperalgesia induced by temporomandibular joint osteoarthritis (TMJOA) in rats.
Methods :Twenty 6-week-old male SD rats were randomly divided into NS group and MIA group．The rat model of TMJOA was established by injection monosodium iodoacetate ( MIA) into the upper cavity of temporomandibular joint (TMJ) ．The changes of pain threshold in the TMJ region of rats after MIA injection were detected by Von Frey．The changes of condyle structure were observed by Hematoxylin-eosin ( HE ) and Safranin O-fast green stains．Histopathological changes of trigeminal ganglion (TG) were observed by HE stains．The expression and distribution of TG NLRP3 protein were detected by immunohistochemistry．Western blot was used to detect the protein levels of NLRP3 and interleukin (IL) -1 β in TG．The mRNA levels of NLRP3，apoptosis-associated speck-like protein (ASC) ，cysteinyl aspartate specific proteinase ( Caspase-1) ，IL-1β and IL-18 in TG were detected by quantitative real-time polymerase chain reaction ( qRT-PCR) .
Results :Compared to saline group，the pain threshold of experimental TMJ osteoarthritis rats decreased (P＜0. 05) ．TMJ and TG showed obvious pathological changes．The protein and mRNA levels of NLRP3 expressed in the tissues of rats in the TMJOA group increased (P ＜0. 05 ) .
Conclusion:NLRP3 inflammasome may be involved in the regulation of hyperalgesia in TMJOA rats.
- Full text:2024072719402813170NLRP3炎症小体在颞下颌...节骨关节炎痛觉过敏中的作用_白苗苗.pdf
