Neurotrophins and IGFs Attenuated Haloperidol-Induced Neuronal Apoptosis in Mouse Cortical Cell Cultures.
- Author:
Hyo Jung KANG
1
;
Kang Jee YOON
;
Byoung Joo GWAG
;
Young Ki CHUNG
;
Jai Sung NOH
Author Information
1. Department of Psychiatry and Behavioral Sciences, Ajou University, School of Medicine, Suwon, Korea.
- Publication Type:Original Article
- Keywords:
Haloperidol;
Apoptosis;
Neuronal death;
BDNF;
NT4/5;
IGF-I;
IGF-II
- MeSH:
Animals;
Apoptosis*;
Brain-Derived Neurotrophic Factor;
Cell Culture Techniques*;
Cell Death;
Coculture Techniques;
DNA Fragmentation;
Haloperidol;
Insulin-Like Growth Factor I;
Insulin-Like Growth Factor II;
Intercellular Signaling Peptides and Proteins;
Mice*;
Movement Disorders;
Neocortex;
Nerve Growth Factors*;
Neurons*;
Somatomedins
- From:Journal of Korean Neuropsychiatric Association
2000;39(5):920-927
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: We examined the effects of neurotrophins and insulin-like growth factors on cell death induced by haloperidol, a typical anti-psychotic agent. METHOD: Neocortices from 14- or 15-daysold fetal mice for neuron-glia co-cultures were used for this experiment. RESULT: Twenty-four hours treatment of mouse cortical cell cultures with 30 M haloperidol-induced wide spread neuronal apoptosis characterized by cell body shrinkage, DNA fragmentation and condensation. Concurrent treatment with growth factors, BDNF, NT4/5, IGF-I and IGF-II, protect the neurons from the haloperidol-induced neuronal apoptosis(HINA) in a dose dependent manner(10-100ng/ml). CONCLUSION: The present study suggests the possibility that haloperidol toxicity can be hampered with growth factors. Further study about the mechanism underlying the protective capacity of the growth factors on HINA may lead to the development of the new protective strategy for tardive dyskinesia.
