A New Gene Regulated by Electroconvulsive Shock in Rat Brains.
- Author:
Sun Ju CHUNG
1
;
Jeong Eun PARK
;
Ung Gu KANG
;
Young Jin KOO
;
Joo Bae PARK
;
Yong Sik KIM
Author Information
1. Department of Psychiatry, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Electroconvulsive shock;
Differential display-PCR;
Gene;
Rat brain
- MeSH:
Adult;
Animals;
Blotting, Northern;
Brain*;
Clone Cells;
Cloning, Organism;
Electroshock*;
Hippocampus;
Humans;
Male;
Mass Screening;
Rats*;
Rats, Sprague-Dawley;
RNA, Messenger
- From:Journal of Korean Neuropsychiatric Association
2000;39(5):928-935
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions. METHODS: ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA differential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus. RESULTS: By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain. CONCLUSION: In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain.
