Effect of Rotundic acid on proliferation，migration and invasion of human lung adenocarcinoma cells
10.19405/j.cnki.issn1000-1492.2022.11.017
- Author:
Caimei Yang
1
;
Jun Shu
1
;
Jiangxia Zheng
1
;
Jilong Shen
2
Author Information
1. Dept of Respiratory Medicine，The Fourth Affiliated Hospital of Anhui Medical University，Hefei 230011
2. The Key Laboratory of Microbiology and Parasitology of Anhui Province，the Key Laboratory of Zoonoses of High Institutions in Anhui，Hefei 230032
- Publication Type:Journal Article
- Keywords:
Rotundic acid;
human lung adenocarcinoma cells;
proliferation;
migration;
invasion;
janus kinase 2;
signal transducer and activator of transcription 3
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1782-1789
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of Rotundic acid (RA) on proliferation，migration and invasion a- bility of human lung adenocarcinoma cells as well as its possible mechanisms．
Methods :Human lung adenocarci- noma A549 and PC9 cells were divided into control group，blank control group，solvent group and 20，40，60，80 μmol / L RA groups．CCK-8 assay and scratch assay were used to detect the proliferation and horizontal migration of human lung adenocarcinoma cells．Transwell migration assay and Transwell invasion assay were used to detect the longitudinal migration and invasion ability of A549 and PC9 cells in each group．The protein expression levels of ja- nus kinase 2 (JAK2) and signal transducer and activator of transcription 3 ( STAT3) in the supernatants of A549 and PC9 cells were detected by ELISA．The mRNA expression levels of JAK2 and STAT3 were detected by RT- PCR. Statistical analysis was made on the differences among groups in each index.
Results :After RA treatment on human lung adenocarcinoma cells ，compared with the control group ，the proliferation activity of A549 and PC9 cells in the experimental groups decreased (P＜0. 05) ，the number of cells crossing polycarbonate membrane and matrix glue decreased (P＜0. 05) ，the expression of JAK2 and STAT3 proteins in cell supernatant decreased (P < 0. 05) ，and the mRNA expression of JAK2 and STAT3 decreased (P＜0. 05) ．The decrease of the above indices was concentration-dependent and had statistical significance (P＜0. 05) ．Compared with the control group，the pro- liferation activity of A549 and PC9 cells in the solvent group showed no significant difference．
Conclusion :RA may inhibit the proliferation，migration and invasion of human lung adenocarcinoma A549 and PC9 cells in vitro， possibly through the inhibition of JAK2 / STAT3 pathway.
- Full text:2024072521162973184铁冬青酸对人肺腺癌细胞增殖、迁移和侵袭能力的影响_杨彩梅.pdf
