The antioxidant transcription factor Nrf2 and Rad51 interact in BLM reduced DNA damage
10.19405/j.cnki.issn1000-1492.2022.11.015
- Author:
Qianqian Xu
1
;
Peiyu Zhang
1
;
Ni Chen
1
Author Information
1. Teaching and Research Section of Nuclear Medicine， School of Basic Medical Sciences，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
Nrf2;
Rad51;
repair of DNA damage;
bleomycin
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1769-1774
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the mechanism of nuclear factor E2-related factor 2 (Nrf2) in bleomycin (BLM) -induced DNA damage of lung cancer cells．
Methods :The fluorescence intensity of tissue reactive oxygen species (ROS) in human lung cancer cells A549 and H1299 was detected by a reactive oxygen species fluorescent probe ( DCFH-DA) ．DNA damage of A549 and H1299 cells treated with 5 μg / ml BLM was detected by Western blot and micronucleus．Then，the Nrf2-low expression transient siRNA and the stably shRNA-transformed lung cancer cells were respectively constructed to detect the DNA damage after BLM．The interaction between Nrf2 and Rad51 in cells was detected by co-immunoprecipitation method.
Results :Under the action of BLM，ROS levels in A549 and H1299 cells increased significantly，and Nrf2 entered the cell nucleus for activation(P＜0. 01) ．Under the effect of BLM，resveratrol ( Res) stimulated A549 and H1299 cells to up-regulate the protein expression of Nrf2 ( P < 0. 01) ，while the expression of DNA damage marker protein γ-H2AX decreased(P＜0. 01) ．On the contrary，after interfering the protein expression of Nrf2 in A549 and H1299 cells with siRNA，the protein expression of γ-H2AX in A549 and H1299 cells was significantly up-regulated under the action of BLM(P＜0. 01) ．Western blot results showed that the expression of homologous recombination repair protein Rad51 and the expression of Nrf2 protein in A549 cells with low Nrf2 expression were significantly down-regulated (P ＜0. 01) ．
Conclusion :The antioxidant transcription factor Nrf2 in tumor cells can interact with Rad51 to regulate the expression of Rad51 and reduce BLM-induced cellular DNA damage.
- Full text:2024072521081202498抗氧化转录因子Nrf2与R...低BLM对细胞DNA的损伤_徐倩倩.pdf
