Effect of teriparatide on osteoblast differentiation in high glucose environment through Wnt3a / β-catenin pathway
10.19405/j.cnki.issn1000-1492.2022.11.012
- Author:
Yingying Gu
1
;
Tian Hou
1
;
Yazhi Qin
1
;
Yan Zhang
1
;
Guochen Wen
1
;
Wenjuan Bi
1
;
Wei Dong
1
Author Information
1. School of Stomatology，North China University of Science and Technology，Tangshan 063210
- Publication Type:Journal Article
- Keywords:
teriparatide;
osteoblasts;
high glucose;
Wnt3a / β-catenin
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1750-1755
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of teriparatide ( TPTD) on the generation of MC3T3-E1 cells to- wards osteogenic differentiation via the Wnt3a / β-catenin pathway in a high-glucose environment．
Methods :The experiment was divided into five groups : low glucose group，low glucose + TPTD group，high glucose group，high glucose + TPTD group，high glucose + TPTD + Wnt3a inhibitor G244-LM group．Cell proliferation activity was de- tected by Calcein-AM and CCK-8 assay，cell mineralized nodule formation was observed by ALP and alizarin red staining，and actin formation was analyzed by immunofluorescence assay. Real-time PCR was performed to detect Wnt3a，β-catenin，Tcf1，OPG and COL Ⅰ mRNA expression.
Results :TPTD had no significant effect on the pro- liferative activity of MC3T3-E1 cells under high glucose condition．The ALP staining area，protein activity and aliza- rin red staining area of the cells in the low glucose + TPTD group were higher than those in the other four groups (P ＜0. 05) ; the high glucose group was lower than the low glucose group (P ＜0. 05 ) ; the high glucose + TPTD group was higher than the high glucose group and the high glucose + TPTD + G244-LM group (P＜0. 05) ．The cy- toskeleton in the low glucose + TPTD group was the clearest ; the cytoskeleton was less clear in both the high glucose and high glucose + TPTD + G244-LM groups than in the high glucose + TPTD group．Genes such as Wnt3a，β-cate- nin，Tcf1，OPG and COL Ⅰ had the highest mRNA levels in the cells of the low glucose + TPTD group (P < 0. 05) ; the mRNA levels of all genes were higher in the low glucose group than thosein the high glucose group (P ＜0. 05) ; the mRNA levels of all genes in the cells of the high glucose + TPTD group were higher than those in the high glucose group and the high glucose + TPTD + G244-LM group ( P＜0. 05) ．
Conclusion :High glucose inhibi- ted osteoblast differentiation，and TPTD promoted osteoblast differentiation in high glucose environment by regula- ting Wnt3a / β-catenin pathway.
- Full text:2024072520570127584特立帕肽通过Wnt3a_β...糖环境下成骨细胞分化的影响_谷营营.pdf
