Comparative proteomics study of sensory and motor fascicles of peripheral nerve
10.19845/j.cnki.zfysjjbzz.2022.0077
- VernacularTitle:比较蛋白质组学在周围神经感觉束和运动束鉴别中作用的实验研究
- Author:
Xianyu MENG
1
;
Laijin LU
1
;
Huanxin CHEN
1
Author Information
1. Department of Orthopedics,The First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China
- Publication Type:Journal Article
- Keywords:
Proteomics;
Differential expression;
Motor fascicles;
Sensory fascicles
- From:
Journal of Apoplexy and Nervous Diseases
2022;39(4):302-305
- CountryChina
- Language:Chinese
-
Abstract:
Objective It is important to distinguish between motor and sensory fascicles of the peripheral nerves for nerve alignment in surgery.No biomarkers currently are available for identification of motor or sensory fascicles.The objective of this study is to search the specific proteins between sensory and motor fascicles of peripheral nerves and provide biomarkers for the identification of functional fascicles of peripheral nerves.Methods The normal state of motor branch and saphenous nerve of femoral nerve in Wistar rats,and at 8 hours and 8 days after Sunderland V injury were respectively sampled.Five mm long samples were taken from the distal side of the broken end,and a total of 18 groups of proteins were isolated from 6 samples.After purification and quantification,differential gel electrophoresis (DIGE) was used to label the proteins,gel image was scanned,and image analysis software (DeCyder) was used to compare and identify the differentially expressed proteins in each group.Protein spots with more than 1.5 times of difference in expression were selected to prepare glue-cutting,enzyme-cutting and spot target.PMF chromatogram was analyzed and identified by MALDI-TOF-PRO mass spectrometer,and the results of proteomics were analyzed and compared by RT-PCR.Chi-square tests and t-tests were performed for comparison between motor or sensory nerve groups.Results The data identified 6 proteins that were differentially expressed between motor and sensory fascicles (>1.5-fold,P<0.05),including Annexin V,neurofilament light polypepticle,TEC kinase,serine protease inhibitor A3N,Peroxiredoxin-2,and TPM1.The proteomic results were consistent with the mRNA expression levels of these genes as determined by quantitative reverse transcription polymerase chain reaction.Conclusion There were significant differences in proteomic expression between the peripheral sensory and motor fascicles,and Annexin V can be used as a high-difference marker protein to distinguish the peripheral sensory from motor fascicles.
- Full text:2024072520493074453Comparative proteomics study of sensory and motor fascicles of peripheral nerve.pdf
