Toxic effects of Mn2+ compound in combination with chloroquine on PC12 cells
10.3969/j.issn.1006-2483.2024.04.004
- VernacularTitle:含Mn2+化合物和氯喹联合作用对PC12细胞株的毒性机制研究
- Author:
Xiaolong FU
1
;
Zijing YU
2
;
Yonggang YANG
1
Author Information
1. Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education，Zunyi Medical University，Zunyi , Guizhou 563006，China
2. Taihe Hospital , Affiliated Hospital of Hubei University of Medicine , Shiyan , Hubei 442000 , China
- Publication Type:Journal Article
- Keywords:
MnCl2;
Chloroquine;
Environmental and food exposure;
Neurotoxicity;
Mitochondrial function
- From:
Journal of Public Health and Preventive Medicine
2024;35(4):16-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the toxicity of low concentration Mn2+ compound combined with autophagy inhibitor chloroquine on nerve cell line PC12 cells for long-term and its mechanism. Methods PC12 cells at logarithmic growth stage were treated with 0 (control), 5, 10, 25, 50, 100, and 200 μM manganous chloride, and 5, 10, 25, 50, and 100 μM chloroquine for 24 h, respectively. The effect of manganous chloride and chloroquine on cell viability was detected by MTT assay. The combined effect of the two compounds on cell viability was determined at 24, 48 and 72 h, respectively. The mitochondrial respiratory function was further examined to explore the possible toxicity mechanism of manganous chloride and chloroquine. Results Compared with the control group, manganous chloride and chloroquine alone had inhibitory effect on cells survival in a concentration-dependent manner. Manganous chloride and chloroquine at concentrations of 40 μM and 2.5 μM, respectively, had no significant effect on cell survival. Compared with the control group, administration of 2.5 μM chloroquine alone for 24, 48 and 72 h did not significantly change cell survival and mitochondrial respiratory function. Treatment of cells with manganous chloride alone at the concentration of 40 μM for 72 h did affect mitochondrial respiratory function. However, the cell survival and mitochondrial respiratory function in the combined administration of manganous chloride and chloroquine for 72 h were significant decreased (P< 0.05). Conclusion The long-term combination of low-concentration manganous chloride and chloroquine produced an additive cytotoxicity on PC12 cells, and the toxicity mechanism may be related to the damage of mitochondrial function.
