The Genetic Polymorphism and Structural Analysis of 47 Microhaplotypes in a Jiangsu Changshu Chinese Han Population
10.16476/j.pibb.2023.0027
- VernacularTitle:江苏常熟地区汉族人群47个微单倍型的遗传多态性及遗传结构分析
- Author:
Kun-Peng PAN
1
;
Yao-Sen FENG
2
;
Wen-Shuai YU
2
;
Zong-Wei LIU
3
;
Yi-Ren YAO
2
;
Jie ZHAO
2
;
Ke-Lai KANG
2
;
Chi ZHANG
2
;
Le WANG
2
;
Jian WU
1
Author Information
1. School of Forensic Medicine, Kunming Medical University, Kunming 650500, China
2. National Engineering Laboratory for Forensic Science, Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China
3. Changshu Public Security Bureau, Changshu 215500, China
- Publication Type:Journal Article
- Keywords:
forensic genetics;
microhaplotype;
next generation sequencing
- From:
Progress in Biochemistry and Biophysics
2024;51(2):423-434
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the genetic polymorphism and structure of 47 autosomal microhaplotypes in the Han population in Changshu City, Jiangsu Province, and to evaluate the forensic efficiencies and forensic parameters. MethodsThe DNA library of unrelated individual samples was prepared according to MHSeqTyper47 kit manual and sequenced on the MiSeq FGx platform. Microhaplotype genotyping and sequencing depth statistics were processed using MHTyper. The genetic information of samples was then evaluated. The fixation index and genetic distance between the Jiangsu Changshu population and the reference populations in the 1000 Genomes Project phase 3 (1KG) were calculated, and forensic parameters were evaluated. ResultsThe fixation index and genetic distance between the Han population in Changshu, Jiangsu, and the CHB (Han Chinese in Beijing, China) reference population in 1KG were the lowest. The effective allele number (Ae) of each locus is also the closest between the two populations. The combined matching probability (CMP) of the Changshu Han population is close to the 5 populations of the East Asian reference super-population in 1KG, which is 1.25×10-36, and the combined probability of exclusion reached 0.999 999 999 964 1. ConclusionThis study reported the genetic polymorphism and allele frequency of 47 microhaplotypes in a Han population in Changshu City, Jiangsu Province. This information provides a data basis for 47 microhaplotypes in forensic applications. In addition, the polymorphism differences between the 1KG reference population and the Han population in Changshu, Jiangsu were compared, and the genetic structure of 47 microhaplotypes in the Han population in Changshu, Jiangsu was revealed. In general, the reference data of the East Asian super-population in 1KG is more in line with the genetic characteristics of Han population in Changshu, Jiangsu.
