Cellular Temperature Imaging Technology Based on Single-molecule Quantum Coherent Modulation
10.16476/j.pibb.2023.0423
- VernacularTitle:基于单分子量子相干调制的细胞温度成像技术
- Author:
Hai-Tao ZHOU
1
;
Cheng-Bing QIN
2
;
Lian-Tuan XIAO
2
;
Zhi-Fang WU
1
;
Si-Jin LI
1
Author Information
1. Department of Nuclear Medicine, The First Hospital of Shanxi Medical University, Collaborative Innovation Center for Molecular Imaging, Shanxi Medical University, Taiyuan 030001, China
2. Collaborative Innovation Center of Extreme Optics, Institute of Laser Spectroscopy, State Key Laboratory of Quantum Optics and Quantum Optics Devices, Shanxi University, Taiyuan 030006, China
- Publication Type:Journal Article
- Keywords:
quantum coherent modulation;
single-molecule microscopy;
cellular temperature imaging
- From:
Progress in Biochemistry and Biophysics
2024;51(5):1215-1220
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveCellular temperature imaging can assist scientists in studying and comprehending the temperature distribution within cells, revealing critical information about cellular metabolism and biochemical processes. Currently, cell temperature imaging techniques based on fluorescent temperature probes suffer from limitations such as low temperature resolution and a limited measurement range. This paper aims to develop a single-cell temperature imaging and real-time monitoring technique by leveraging the temperature-dependent properties of single-molecule quantum coherence processes. MethodsUsing femtosecond pulse lasers, we prepare delayed and phase-adjustable pairs of femtosecond pulses. These modulated pulse pairs excite fluorescent single molecules labeled within cells through a microscopic system, followed by the collection and recording of the arrival time of each fluorescent photon. By defining the quantum coherence visibility (V) of single molecules in relation to the surrounding environmental temperature, a correspondence between V and environmental temperature is established. By modulating and demodulating the arrival times of fluorescent photons, we obtain the local temperature of single molecules. Combined with scanning imaging, we finally achieve temperature imaging and real-time detection of cells. ResultsThis method achieves high precision (temperature resolution<0.1°C) and a wide temperature range (10-50°C) for temperature imaging and measurement, and it enables the observation of temperature changes related to individual cell metabolism. ConclusionThis research contributes to a deeper understanding of cellular metabolism, protein function, and disease mechanisms, providing a valuable tool for biomedical research.
