miR-375 Attenuates The Migration and Invasion of Osteosarcoma Cells by Targeting MMP13
10.16476/j.pibb.2023.0391
- VernacularTitle:miR-375靶向MMP13抑制骨肉瘤细胞的迁移和侵袭
- Author:
Zhong LIU
1
;
Lei HE
1
;
Jian XIAO
1
;
Qing-Mei ZHU
1
;
Jun XIAO
1
;
Yong-Ming YANG
1
;
Yong-Jian LUO
1
;
Zhong-Cheng MO
1
;
Yi-Qun ZHANG
1
;
Ming LI
2
Author Information
1. Department of Orthopedics, the First Affiliated Hospital of Shaoyang University, Shaoyang 422000, China
2. School of Basic Medicine, Hunan University of Medicine, Huaihua 418000, China
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
miR-375;
MMP13;
migration;
invasion
- From:
Progress in Biochemistry and Biophysics
2024;51(5):1203-1214
- CountryChina
- Language:English
-
Abstract:
ObjectiveTo explore whether miR-375 regulates the malignant characteristics of osteosarcoma (OS) by influencing the expression of MMP13. MethodsPlasmid DNAs and miRNAs were transfected into OS cells and HEK293 cells using Lipofectamine 3000 reagent. Real-time quantitative polymerase chain reaction was performed to measure the expression of miR-375 and MMP13 in OS patients and OS cells. Western blot was performed to analyze the MMP13 protein in the patients with OS and OS cells. The targeting relationship between miR-375 and MMP13 was analyzed by luciferase assay. Migration and invasion were analysed by heal wound and transwell assays, respectively. ResultsmiR-375 expression in OS tissues was lower than that in normal tissues. The expression of MMP13 was upregulated in OS tissues. MMP13 expression was negatively correlated withmiR-375 expression in patients with OS. Migration and invasion were significantly inhibited in OS cells with the miR-375 mimic compared with OS cells with the miRNA control. MMP13 partially reversed the inhibition of migration and invasion induced by miR-375 in the OS cells. ConclusionmiR-375 attenuates migration and invasion by downregulating the expression of MMP13 in OS cells.
