Impacts of cichoric acid on neuronal apoptosis and inflammatory response in ischemic stroke rats by regulating p38 MAPK/NF-κB/NLRP3 signaling pathway
10.19845/j.cnki.zfysjjbzz.2022.0175
- VernacularTitle:菊苣酸调控p38 MAPK/NF-κB/NLRP3信号通路对缺血性脑卒中大鼠神经元凋亡及炎症反应的影响
- Author:
Fuhui ZHANG
1
;
Jingfeng HAO
1
;
Ziyan ZHANG
1
Author Information
1. Department of Neurology,Handan Second Hospital,Handan 056001,China
- Publication Type:Journal Article
- Keywords:
Cichoric acid;
Ischemic stroke rats;
Neuronal apoptosis;
Inflammatory response;
P38 mitogen activated protein kinase/nuclear factor-κB/nucleotide binding oligomerization domain like receptor protein 3 signaling pathway
- From:
Journal of Apoplexy and Nervous Diseases
2022;39(8):694-698
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the effects and mechanism of cichoric acid (CA) on neuronal apoptosis and inflammatory response in ischemic stroke rats.Methods The ischemic stroke rat model was prepared by the modified suture method.The rats were grouped into sham operation group,model group,CA group (10 mg/kg),and CA+p38 MAPK activator (Anisomycin) group (10 mg/kg CA+2 mg/kg Anisomycin).Each group received corresponding intervention for 2 weeks,the Zea Longa score were performed and volume percentage of cerebral infarction were detected on rats,Nissl staining was used to detect neuronal damage in rat hippocampal tissue.TUNEL method was used to detect neuronal apoptosis in rat hippocampal tissue,the levels of tumor necrosis factor-α (TNF-α),interleukin (IL)-1β and IL-6 in rat hippocampal tissue were detected by enzyme-linked immunosorbent assay,the expression of phosphorylated p38 MAPK (p-p38 MAPK),p38 mitogen activated protein kinase (p38 MAPK),phosphorylated NF-κB p65 (p-NF-κB p65),nuclear factor-κB p65 (NF-κB p65),nucleotide binding oligomerization domain like receptor protein 3 (NLRP3) protein in rat hippocampal tissue was detected by Western blot.Results Compared with the sham operation group,the morphology of neurons in the model group was irregular,the staining was shallow,the number of Nissl bodies decreased significantly,and the Zea Longa score,the volume percentage of cerebral infarction,the rate of neuronal apoptosis and the TNF-α,IL-1β,IL-6,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65,NLRP3 protein expression leves in hippocampal tissue increased significantly (P<0.05);compared with the model group,the morphology of neurons in CA group was significantly improved,the morphology was plump,the staining was uniform,the Nissl body was dense,and the Zea Longa score,the volume percentage of cerebral infarction,the rate of neuronal apoptosis and the TNF-α,IL-1β,IL-6,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65,NLRP3 protein expression leves in hippocampal tissue decreased significantly (P<0.05);Anisomycin can reverse the above-mentioned improvement effect of CA on ischemic stroke rats.Conclusion CA can inhibit the inflammatory response,reduce neuronal apoptosis,and alleviate the brain injury of rats caused by ischemic stroke by inhibiting the p38 MAPK/NF-κB/NLRP3 signaling pathway.
- Full text:2024072322353602616Impacts of cichoric acid on neuronal apoptosis and inflammatory response in ischemic stroke rats by regulating p38 MAPK_NF-κB_NLRP3 signaling pathway.pdf
