Role of mitochondrial autophagy in NLRP3 mediated inflammatory response in stroke rehabilitation
10.19845/j.cnki.zfysjjbzz.2022.0174
- VernacularTitle:线粒体自噬参与NLRP3介导的炎症反应在脑卒中康复中的作用
- Author:
Wei WANG
1
;
Zhendong LI
1
;
Chengcheng ZHANG
1
Author Information
1. Graduate School of Hunan University of Traditional Chinese Medicine,Changsha 410000,China
- Publication Type:Journal Article
- Keywords:
Mitochondrial autophagy;
NLRP3;
Ischemia/reperfusion;
Rat;
Neuron;
Microglia
- From:
Journal of Apoplexy and Nervous Diseases
2022;39(8):689-693
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the role of mitochondrial autophagy and NLRP3 mediated inflammatory response in stroke rehabilitation.Methods Male SD rats were randomly divided into 6 groups:sham operation group (sham),sham+rapamycin (rapa) group,6 hours after reperfusion (i/r 6 h),i/r 6 h+Rapa group,24 hours after reperfusion (i/r 24 h) and i/r 24 h+Rapa group.A transient middle cerebral artery occlusion model was established to stimulate ischemia/reperfusion (i/r) injury in rats.The expression of caspase-1 positive cells in different types of neurons in ischemic core cortex was analyzed.The expression of NLRP3 and mitochondrial membrane potential were measured in BV2 cells under hypoxia glucose deprivation/reoxygenation (ogd/r).Results At 6 h after i/r injury,cleaved caspase-1 was mainly expressed in microglia (88.4±1.1)% and neurons (63.4±2.2)% at 24 h.After ogd/r,the transformation from lc3-Ⅰ to lc3-Ⅱ in BV2 cells decreased with time.BV2 cells showed a percentage of low membrane potential 24 hours after exposure to ogd/r.Compared with ogd/r group,Rapa could rescue mitochondrial damage (P<0.05),and Rapa could inhibit NLRP3,cleaved caspase-1 and cleaved IL-1 in BV2 cells induced by ogd/r β.The expression level was up-regulated (P<0.05).In the i/r+rapa group,the expression of cleaved caspase-1 in microglia ［(12.7±1.8)% vs (70.0±2.0)%］ and neurons ［(11.9±1.9)% vs (70.8±1.7)%］ was significantly lower than that in the i/r group at 6 or 24 hours after brain i/r injury (P<0.001).Conclusion Down regulation of mitochondrial autophagy is essential for the activation of NLRP3 inflammasomes in microglia,and an autophagy inducer can effectively alleviate the NLRP3 inflammasome response in microglia,and neurons after OGD/R and cerebral I/R injury.
- Full text:2024072322320711840Role of mitochondrial autophagy in NLRP3 mediated inflammatory response in stroke rehabilitation.pdf
