Qiyu Sanlong Decoction enhances Th1 immune response through PD-1 signaling pathway and inhibits metastasis of lung cancer
10.19405/j.cnki.issn1000-1492.2022.11.007
- Author:
Annan Jiao
1
;
Huan Wu
2
;
Jie Zhu
3
;
Mei Zhang
1
;
Ping Li
1
;
Zegeng Li
4
;
Yang Chen
1
Author Information
1. Dept of Integrated Traditional Chinese and Western Medicine Oncology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
2. Scientific Research ＆ Experiment Center，Anhui University of Chinese Medicine， Hefei 230038
3. Dept of Integrated Traditional Chinese and Western Medicine Oncology，Anhui University of Chinese Medicine， Hefei 230038
4. The First Affiliated Hospital of Anhui University of Chinese Medicine，Hefei 230601
- Publication Type:Journal Article
- Keywords:
lung cancer;
Qiyu Sanlong Decoction;
PD-1;
Th1;
metastasis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1718-1724
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of Qiyu Sanlong Decoction ( QYSLD) on inhibiting lung cancer by regulating tumor immune microenvironment through PD-1 signaling pathway and on Th1 immune response in lung cancer bearing mice．
Methods :Lewis lung cancer cell line ( LLC) was used to model subcutaneously implanted tumor in mice．72 mice were randomly divided into Control group ( n = 18 ) ，cisplatin group ( n = 18 ) ，QYSLD group (n = 18) and combined group (n = 18) ．The general survival of mice was observedand recorded ; the specific Th1 cell response to tumor antigen was detected by ELISA method ; the proportion of PD-1 positive cells and the ap- optosis of memory CD4 + T cells in spleen T cells of mice were detected by flow cytometry ; and the number and vol- ume of pulmonary metastatic nodules were counted under an anatomical microscope after 21 days of continuous treatment.
Results :QYSLD could improve the general survival of mice bearing lung cancer，up-regulate the im- mune response of CD4 + T cells and the specific killing effect of CD8 + T cells．The proportion of apoptotic cells in PD-1 positive cells and CD4 + memory T cells in spleen T cells of mice was down-regulated，and lung metastasis of mouse tumor cells was inhibited．
Conclusion :QYSLD can improve the survival status of mice bearing lung cancer and enhance Th1 immune response by mediating PD-1 signaling pathway，improve immune function and inhibit im- mune escape of tumor cells，thus inhibiting the metastasis of lung cancer.
- Full text:2024072322194610358芪玉三龙汤通过PD-1信号...Th1免疫反应抑制肺癌转移_焦安男.pdf
