Effect of ABCA1 on epithelial-mesenchymal transition in gastric cancer by activating the EGFR / STAT3 signaling pathway
10.19405/j.cnki.issn1000-1492.2022.11.005
- Author:
Yong Jin
1
,
2
;
Hong Du
1
;
Ying Xia
2
,
3
;
Tingting Xiang
2
;
Sixi Wei
1
,
2
;
Ming Sun
2
;
Hai Huang
1
,
2
Author Information
1. Center for Clinical Laboratories，The Affiliated Hospital of Guizhou Medical University，Guiyang 550004
2. School of Clinical Laboratory Science，Guizhou Medical University，Guiyang 550004
3. Dept of Clinical Laboratory， The First Hospital Attached to Guizhou University of Traditional Chinese Medicine，Guiyang 550001
- Publication Type:Journal Article
- Keywords:
gastric cancer;
ABCA1;
invasion;
migration;
epithelial-mesenchymal transition
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1705-1711
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effect of ATP binding cassette subfamily A member 1 (ABCA1) on the inva- sion，migration and epithelial-mesenchymal transition(EMT) of human gastric cancer and investigate the underlying mechanism．
Methods :The expression of ABCA1 in human gastric cancer and its relationship with the prognosis of gastric cancer patients were analyzed by Bioinformatics analysisbased on network databases. RT-qPCR was per- formed to detect the expression of ABCA1 in 25 pairs of clinical gastric cancer tissue samples ; RT-qPCR and West- ern blot were used to analyze the expression of ABCA1 in different human gastric cancer cell lines ; Gene silencing technology was used to construct stable low-expressing ABCA1 gastric cancer cell line．Cell migration and invasion were determined by transwell assay ; Western blot was used to detect the expression of related marker proteins in EMT and epidermal growth factor receptor (EGFR) / signal transducer and activator of transcription(STAT3) signa- ling pathway.
Results :The results of bioinformatics analysis indicated that ABCA1 was highly expressed in gastric cancer，which was positively correlated with advanced grade and stage as well as the poor prognosis of gastric canc- er patients. RT-qPCR results showed that ABCA1 was highly expressed in gastric cancer tissues and gastric cancer cells HGC-27 ; The results of Transwell indicated that knockdown of ABCA1 could inhibit the invasion and migra- tion of HGC-27 cells．Western blot results showed that the expression of mesenchymal cell marker proteins (Vimen- tin，N-Cadherin) and EMT-related transcription factors (Twist，Zeb1 and Snail) in the ABCA1 knockdown group was down-regulated，while the expression of epithelial cell marker protein E-Cadherin was up-regulated．Western blot results showed that compared with the control group，knockdown of ABCA1 inhibited the expression of EGFR downstream molecule STAT3，and reduced the phosphorylation level of EGFR and its downstream molecule STAT3 .
Conclusion :ABCA1 is up-regulated in gastric cancer and is correlated with cell invasion，migration，EMT and in- dicate poor prognosis of gastric cancer patients．ABCA1 may affect the expression and activation of STAT3 by regu- lating EGFR , thereby inhibiting the invasion，migration and EMT of gastric cancer cells.
- Full text:2024072322093836587ABCA1通过激活EGFR...路对胃癌上皮间质转化的影响_金泳.pdf
