Screening of genes related to proliferation of gastric cancer cells based on CRISPR / dCas9-SAM system
10.19405/j.cnki.issn1000-1492.2022.11.003
- Author:
Yu Peng
1
,
2
;
Qifan Gong
2
;
Fumin Tai
2
;
Tiantian Wang
2
;
Changhui Ge
2
;
Xiaofei Zheng
2
;
Yide Qin
1
;
Hanjiang Fu
1
,
2
Author Information
1. School of Basic Medicine，Anhui Medical University，Hefei 230032
2. Beijing Key Laboratory for Radiobiology，Institute of Radiation Medicine，Academy of Military Medical Science，Beijing 100850
- Publication Type:Journal Article
- Keywords:
CRISPR / dCas9-SAM system;
cell proliferation;
gastric carcinoma;
long non-coding RNA
- From:
Acta Universitatis Medicinalis Anhui
2022;57(11):1693-1698
- CountryChina
- Language:Chinese
-
Abstract:
Objective :The CRISPR / dCas9-SAM system was used to explore genes related to the proliferation of gastric cancer cells AGS，and their role in the occurrence and development of gastric cancer was analyzed．
Methods :sgRNA was designed for genes with differential expression between gastric cancer and normal gastric tissue， and a lentiviral library was obtained after packaging was constructed．The AGS cells at different time points after the library was infected with AGS cells were used as the screening pressure，and the AGS cells at three time points on days 0，7 and 14 were collected．High-throughput sequencing analyzed sgRNA enrichment in AGS cells at dif- ferent time points after infection to obtain differential genes related to AGS cell proliferation.
Results :Bioinformat- ics showed that compared with the 0 d group，42 and 45 negative screening differential genes and 59 and 40 posi- tive screening differential genes were obtained in the 7 d group and 14 d group，respectively．Among them，the 7 d group and the 14 d group had 11 genes in the negative screening and the positive screening．
Conclusion :In this study，11 genes inhibiting the proliferation of AGS cells were screened，of which 5 were protein-coding genes and 6 were long non-coding RNA ( lncRNA ) genes． 11 candidate genes that promoted AGS cell proliferation were screened，of which 3 were protein-coding genes and 8 were lncRNA genes．It laid a foundation for further function- al verification and comprehensive analysis of the occurrence and development process of gastric cancer.
- Full text:2024072321543929858基于CRISPR_dCas...统筛选胃癌细胞增殖相关基因_彭玉.pdf
