LINC01116 aggravates oxygen-glucose deprivation/reoxygenation-induced injury in human hippocampal astrocytes by targeting miR-203
10.19845/j.cnki.zfysjjbzz.2022.0221
- VernacularTitle:LINC01116靶向miR-203加剧氧糖剥夺/复氧诱导下人海马星形胶质细胞损伤
- Author:
Zhengyi HUANG
1
;
Zengxia ZHAO
1
;
Qiuhong FU
1
Author Information
1. Department of Geriatrics,Shenzhen Longhua District Central Hospital,Shenzhen 518110,China
- Publication Type:Journal Article
- Keywords:
LINC01116;
AIS;
hHA;
OGD/R;
Therapeutic target
- From:
Journal of Apoplexy and Nervous Diseases
2022;39(10):887-892
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role and molecular mechanism of long intergenic non-coding RNA 1116 (LINC01116) in hippocampal astrocytes of acute ischemic stroke (AIS).Methods The expressions of LINC01116 and miR-203 in serum of 131 AIS patients before and after thrombolysis were detected by real-time fluorescence quantitative PCR.The regulatory effect of LINC01116 on miR-203 was detected by dual-luciferase reporter gene and RNA-binding protein immunoprecipitation assay.Human hippocampal astrocytes (hHA) were applied to establish an oxygen-glucose deprivation/reoxygenation (OGD/R) model and were treated with LINC01116 interference and LINC01116 combined with miR-203 interference.The changes of cell proliferation,cell apoptosis,production of reactive oxygen species (ROS),activity of lactate dehydrogenase (LDH),and inflammatory factors were detected by CCK-8,TUNEL and Western blotting,ROS assay,LDH assay,and enzyme-linked immunosorbent assay,respectively.Results The expressions of LINC01116 and miR-203 in serum after thrombolysis were higher and lower than those before thrombolysis,respectively,and the expressions of LINC01116 and miR-203 were negatively correlated (P<0.05).LINC01116 inhibited miR-203 expression by sponge of miR-203 (P<0.05).LINC01116 interference alleviated the OGD/R-induced injury of hHA cells,which manifested as elevated cell proliferation ability,decreased cell apoptosis rate,decreased protein expressions of cleaved caspase-3 and Bax but raised protein expression of Bcl-2,reduced ROS production,decreased LDH activity,and downregulated TNF-α,IL-1β and IL-6 concentrations but upregulated IL-4,IL-10 and IL-13 concentrations (P<0.05).miR-203 interference reversed the protective effect of LINC01116 interference on the OGD/R-induced injury of hHA cells (P<0.05).Conclusions LINC01116 promotes the OGD/R-induced injury of hHA cells by targeting miR-203,suggesting that the LINC01116/miR-203 pathway might be a potential therapeutic target for AIS.
- Full text:2024072320432985785LINC01116 aggravates oxygen-glucose deprivation_reoxygenation-induced injury in human hippocampal astrocytes by targeting miR-203.pdf
