Changes in serum miR-320a and AQP4 levels in patients with Alzheimer’s disease and their clinical significance
10.19845/j.cnki.zfysjjbzz.2022.0219
- VernacularTitle:阿尔茨海默病患者血清miR-320aAQP4水平变化及临床意义
- Author:
Yamin YUE
1
;
Suyan GUO
1
;
Yubo YANG
1
Author Information
1. Neurology Department,Puyang Oilfield General Hospital,Puyang 457000,China
- Publication Type:Journal Article
- Keywords:
Alzheimer’s disease;
Amyloid-β;
MicroRNA-320a;
Aquaporin 4;
Cognition
- From:
Journal of Apoplexy and Nervous Diseases
2022;39(10):877-881
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes and clinical significance of serum microRNA-320a (miR-320a) and aquaporin 4 (AQP4) levels in patients with Alzheimer’s disease (AD).Methods Eighty patients with AD admitted to Puyang Oilfield General Hospital from January 2016 to April 2022 were selected as the AD group,and another 34 healthy people with physical examination in the same period were selected as the control group.qRT-PCR was used to detect serum miR-320a levels,and ELISA was used to detect serum amyloid-β (Aβ) 42,Aβ40,Aβ42/Aβ40,AQP4 levels,and the simple mental state examination (MMSE) was used to assess cognitive function.The relationship of serum miR-320a and AQP4 levels with Aβ42,Aβ40,Aβ42/Aβ40 levels and MMSE score in AD patients,and their diagnostic value for AD were analyzed.Results Compared with the control group,serum Aβ42,Aβ42/Aβ40,miR-320a,AQP4 levels and MMSE scores were reduced and serum Aβ40 levels were increased in the AD group (P<0.05).Pearson/Spearman correlation analysis showed that serum miR-320a and AQP4 levels in AD patients were positively correlated with Aβ42,Aβ42/Aβ40 levels and MMSE scores,and negatively correlated with Aβ40 levels (P<0.05).The analysis of the working characteristic curve of subjects showed that the AUC of serum mir-320a and AQP4 levels in the diagnosis of AD alone and in combination were 0.802,0.819 and 0.913 respectively,the sensitivity was 83.75%,87.50% and 92.50% respectively,and the specificity was 70.59%,70.59% and 82.35% respectively.The combined diagnosis of the two can further improve the diagnostic value (P<0.05).Conclusion Serum miR-320a and AQP4 levels are reduced in AD patients,both of which are associated with Aβ deposition and cognitive function in AD patients and can be used as auxiliary diagnostic indicators of AD.
- Full text:2024072320373160449阿尔茨海默病患者血清miR-320aAQP4水平变化及临床意义.pdf
