Rapid detection of Salmonella by enzymatic recombinase amplification combined with lateral flow chromatography
10.19485/j.cnki.issn2096-5087.2023.12.020
- Author:
NIE Yanni
;
YAN Meiying
;
SONG Yanyan
- Publication Type:Journal Article
- Keywords:
enzymatic recombinase amplification;
lateral flow;
Salmonella;
point of care testing
- From:
Journal of Preventive Medicine
2023;35(12):1102-1104
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rapid detection method for Salmonella based on the combination of enzymatic recombinase amplification (ERA) and lateral flow chromatography (LF), so as to provide technical support for the on-site detection of Salmonella.
Methods:Specific ERA primers and probes were designed based on the highly conserved flagella gene fimY in Salmonella. The primers were screened using capillary electrophoresis, and the probes were designed according to the amplification range of the screened primers. The amplification temperature and time were optimized to establish the amplification method, and the product was detected using LF strips. A standard strain of Salmonella was used to verify the sensitivity, 10 other gut bacteria were used to to verify the specificity and sensitivity, and the nucleic acid of the actual Salmonella strains was amplified to verify the detectability.
Results:After screening for Salmonella-specific primers using capillary electrophoresis, the minimum detection concentration was 5 copies/μL under the amplification temperature of 37 ℃ and reaction time of 20 minutes. This method had a positive amplification result for Salmonella nucleic acid, and the amplification results of 10 other gut bacteria were all negative, with good specificity.
Conclusion:This method provides a possibility for on-site point of care testing of Salmonella infection.
- Full text:2024072310474871150酶促重组酶扩增-侧流层析快速检测沙门菌的方法研究.pdf
