hsa －let－7e－5p inhibits the migration of lung adenocarcinoma cells
10.19405/j.cnki.issn1000-1492.2022.12.020
- Author:
Xiao Feng
1
;
Yun Li
1
;
Fan Bu
2
;
Fengsong Wang
3
;
Quan Wu
1
Author Information
1. Dept of Clinical Laboratory，Provincial Hospital Affiliated to Anhui Medical University，Hefei 230001
2. School of Life Sciences，USTC Life Sciences and Medicine，Hefei 230027
3. Dept of Biology，School of Life Sciences，Anhui Medical University，Hefei 230032
- Publication Type:Journal Article
- Keywords:
hsa-let-7e-5p;
lung adenocarcinoma;
miRNA microarray;
tumor cell migration
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1964-1970,1978
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To screen differentially expressed miRNAs and explore its effect and mechanism on cell mi- gration in lung adenocarcinoma (LUAD) ．
Methods :Differentially expressed miRNAs in LUAD tissues and normal lung tissues were screened by miRNA microarrays，and then bioinformatics analysis was used to predict their poten- tial biological functions and signaling pathways．The cancer genome atlas (TCGA) analysis and quantitative real- time PCR (qRT-PCR) verified the expression level of hsa-let-7e-5p in LUAD tissues and cell lines．The effect of hsa-let-7e-5p cell migration in LUAD was examined by would healing experiment．After screening the underlying target genes by bioinformatics analysis ，the targeting relationship between hsa-let-7e-5p and DTX2，NME6， C8orf58，GATM and DHX57 were verified by qRT-PCR.
Results :The miRNA microarray results showed that 347 miRNAs were down-regulated while 229 miRNAs were up-regulated in lung adenocarcinoma tissues．Compared with normal lung tissue and cells，the expression level of hsa-let-7e-5p was significantly down-regulated．Besides，over- expression of hsa-let-7e-5p inhibitedLUAD cell migration．
Conclusion :Non-coding RNA hsa-let-7e-5p is down- regulated in LUAD and inhibits the migration of lung adenocarcinoma cells．DTX2，NME6，C8orf58，GATM and DHX57 are the potential target genes of hsa-let-7e-5p.
- Full text:2024072222035916140hsa-let-7e-5p对肺腺癌细胞迁移的影响_冯笑.pdf
