Study on effect and mechanism of HIF －1 α silencing combined with methylselenenic acid on proliferation and apoptosis of cervical cancer cells
10.19405/j.cnki.issn1000-1492.2022.12.018
- Author:
Zhenhao Li
1
;
Yaofeng Hou
1
;
Ling Li
1
;
Xuanyu Huang
1
;
Wanyu Liu
1
;
Aixia Zhang
2
;
Nan Wang
1
Author Information
1. School of Life Sciences，Jiaying University，Meizhou 514015
2. Agro-biological Gene Research Center，Guangdong Academy of Agricultural Sciences，Guangzhou 510640
- Publication Type:Journal Article
- Keywords:
hypoxia inducer factor-1α;
methylselenenic acid;
cervical cancer;
cell proliferation;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1954-1959
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the influence and molecular mechanism of hypoxia-inducing factor-1 α( HIF- 1 α) gene silencing combined with methyl selenenic acid (MSA) on cervical cancer cell proliferation，apoptosis and cell migration．
Methods :HeLa cells were transfected with HIF-1 interference RNA and negative control RNA．Af- ter transfection for 48 h，cells were stimulated with MSA for 24 h，and cell proliferation was determined by CCK-8 assay and colony formation．Apoptosis was determined by flow cytometry combined with Annexin V-FITC / PI．The expression levels of HIF-1α , Bcl-2 ，and E-cadherin were detected by Western blot assay． Cell migration ability was determined by Transwell assay. RNA-seq analysis was used to investigate the differentially expressed genes and differential signaling pathways.
Results :Compared with the control group，interfering with HIF-1α combined with MSA significantly inhibited cell proliferation (P ＜0．01) ．Flow cytometry results showed that the combined drug group significantly induced apoptosis．Transwell results showed that interfering with HIF-1α combined with MSA
inhibited HeLa cell migration．Compared with the control group，interfering with HIF-1α combined with MSA down- regulated the expression of Bcl-2 and up-regulated the expression of E-cadherin. RNA-sequencing combined with signal pathway enrichment results showed that the expression of apoptotic signal pathway and downstream genes was inhibited．
Conclusion :HIF-1α gene silencing combined with MSA can synergically inhibit the proliferation and induce apoptosis of cervical cancer cells，and its regulatory mechanism may be related to the expression of Bcl-2 family proteins and the inhibition of p53 signaling pathway.
- Full text:2024072221495332630HIF-1α沉默联合甲基硒...殖与凋亡的影响及其机制探究_李振豪.pdf
