Analysis on the association between dust exposure and pharyngeal microbiota in coal miners based on 16sRNA high-throughput sequencing technology
10.20001/j.issn.2095-2619.20240410
- VernacularTitle:基于16sRNA高通量测序技术分析粉尘接触与煤矿工人咽部菌群变化关联性
- Author:
Xuechun ZHANG
1
;
Yuan WANG
;
Hailan HE
;
Fuhai SHEN
;
Hongli WANG
;
Heliang LIU
Author Information
1. School of Public Health, North China University of Science and Technology, Tangshan, Hebei 063210, China
- Publication Type:Journal Article
- Keywords:
Dust;
Coal miners;
Pharyngeal microbiota;
16sRNA;
High-throughput sequencing
- From:
China Occupational Medicine
2024;51(2):177-182
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the distribution of pharyngeal microbiota in coal miners exposed to dust. Methods Eight coal miners who had been engaged in occupational dust exposure for more than 20 years were selected as the dust-exposed group, and four coal miners who were not exposed to dust at work were selected as the control group using the judgment sampling method. Pharyngeal secretions of the coal miners were collected with throat swabs, and its pharyngeal microbiota was analyzed. The diversity, abundance and evenness of the microbiota were analyzed by gene sequencing using the 16sRNA gene high-throughput sequencing technology. Results A total of 254 operational taxonomic units of pharyngeal microbiota were detected in the coal miners in the control group, which was 210 more than that in the dust-exposed group. The Chao1 index, Shannon index, PD-tree index and Pielou index of pharyngeal microbiota in the dust-exposed group decreased compared with the control group (all P<0.01). The abundance of Bacteroidetes and Clostridum, at the phylum level, in the pharynx of coal miners in the dust-exposed group was higher than that in the control group (all P<0.05). The abundance of Prevotella, Neisseria, and Monas, at the genus level, in the pharynx of coal miners in the dust-exposed group was higher than that in the control group(all P<0.05), while the abundance of Lactobacillus decreased (P<0.05). The analysis results of the receiver operating characteristic curve showed that Lactobacillus, Fusobacterium and Rothia may play a role for pharyngeal microbiota imbalance prediction in dust-exposed workers, and the area under the curves were all 1.00±0.00. Conclusion The species diversity and evenness of pharyngeal microbiota in coal miners exposed to dust are decreased, which may be related to the continuous inhalation of coal dust that disrupts the microbial environment of the throat.
