Effect of silencing MFGE8 on proliferation，apoptosis，invasion， and migration of osteosarcoma cells U2OS
10.19405/j.cnki.issn1000-1492.2022.12.013
- Author:
Yang Li
1
;
Mingxuan Ruan
1
;
Zhen Wang
2
;
Xianglu Zhang
2
;
Fei Huang
1
Author Information
1. Dept of Orthopedics，The Second Affiliated Hospital of Anhui Medical University，Hefei 230601
2. Dept of Orthopedics，The Fourth Affiliated Hospital of Anhui Medical University，Hefei 230012
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
MFGE8;
proliferation;
apoptosis;
invasion;
migration
- From:
Acta Universitatis Medicinalis Anhui
2022;57(12):1921-1926
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of milk fat globule epidermal growth factor 8 protein(MFGE8) in osteosarcoma cell lines and its effects on the proliferation，apoptosis，invasion and migration ability of osteosarco- ma cells(U2OS) ．
Methods :The expression of MFGE8 protein in normal osteoblasts and osteosarcoma cell lines was examined by Western blot method．The relationship between MFGE8 mRNA expression and prognosis of osteo- sarcoma patients was analyzed by GEO database．The effects of down-regulation of MFGE8 on proliferation，apopto- sis，migration and invasion of U2OS cells were examined.
Results :Western blot method showed that the expres- sion of MFGE8 protein was higher in osteosarcoma cells than that in normal osteoblasts and its was negatively corre- lated with the prognosis of osteosarcoma patients ( all P ＜0. 05) ; compared with sh-Ctrl ( U2OS cells transfected with the null group) group，the U2OS cells in sh-MFGE8 (U2OS cells transfected with sh-MFGE8) group had de- creased healing rate and invasion number while increased proliferation inhibition rate and apoptosis rate ，and all differences were statistically significant (P＜0. 05) ．
Conclusion :MFGE8 is highly expressed in U2OS ofosteosar- coma cells，and down-regulation of MFGE8 can inhibit the proliferation，invasion and migration of U2OS and in- duce their apoptosis.
- Full text:2024072116243082537沉默MFGE8对骨肉瘤细胞...殖、凋亡、侵袭及迁移的影响_李阳.pdf
